Lee, Jingu Kim, Joon-Goon Hong, Sujung Kim, Young Seo Ahn, Soyeon Kim, Ryul Chun, Heejung Park, Ki Duk Jeong, Yong Kim, Dong-Eog Lee, C. Justin Ku, Taeyun Kim, Pilhan 2024-01-19T12:03:28Z 2024-01-19T12:03:28Z 2022-04-05 2022-05 0894-1491 https://pubs.kist.re.kr/handle/201004/115259 Cerebral microinfarct increases the risk of dementia. But how microscopic cerebrovascular disruption affects the brain tissue in cellular-level are mostly unknown. Herein, with a longitudinal intravital imaging, we serially visualized in vivo dynamic cellular-level changes in astrocyte, pericyte and neuron as well as microvascular integrity after the induction of cerebral microinfarction for 1 month in mice. At day 2-3, it revealed a localized edema with acute astrocyte loss, neuronal death, impaired pericyte-vessel coverage and extravascular leakage of 3 kDa dextran (but not 2 MDa dextran) indicating microinfarction-related blood-brain barrier (BBB) dysfunction for small molecules. At day 5, the local edema disappeared with the partial restoration of microcirculation and recovery of pericyte-vessel coverage and BBB integrity. But brain tissue continued to shrink with persisted loss of astrocyte and neuron in microinfarct until 30 days, resulting in a collagen-rich fibrous scar surrounding the microinfarct. Notably, reactive astrocytes expressing glial fibrillary acidic protein (GFAP) appeared at the peri-infarct area early at day 2 and thereafter accumulated in the peri-infarct until 30 days, inducing glial scar formation in cerebral cortex. Our longitudinal intravital imaging of serial microscopic neurovascular pathophysiology in cerebral microinfarction newly revealed that astrocytes are critically susceptible to the acute microinfarction and their reactive response leads to the fibrous glial scar formation. English John Wiley & Sons Inc. Longitudinal intravital imaging of cerebral microinfarction reveals a dynamic astrocyte reaction leading to glial scar formation Article 10.1002/glia.24151 1 GLIA, v.70, no.5, pp.975 - 988 GLIA 70 5 975 988 N scie scopus 000749503500001 2-s2.0-85124000697 Neurosciences Neurosciences & Neurology Article; Early Access IN-VIVO CORTICAL MICROINFARCTS FIBROTIC SCAR 3T MRI BRAIN VISUALIZATION FLUORESCENCE QUANTITATION MICROSCOPY ISCHEMIA blood-brain barrier cerebral microinfarct glial scar formation intravital imaging reactive astrocyte