Park, Suin Cho, Kyungjin Lee, Taeho Lee, Eunsu Bae, Hyokwan 2024-01-19T12:03:31Z 2024-01-19T12:03:31Z 2022-04-03 2022-05 0048-9697 https://pubs.kist.re.kr/handle/201004/115261 Partial nitritation (PN) is a bioprocess that is essential for developing cost-effective biological nitrogen removal processes. Understanding the abundant bacterial communities responsible for nitrification under salt stress conditions is important to achieve a stable PN system for treating saline wastewater. Therefore, in this study, we identified the core nitrifying communities and investigated their correlations with the process parameters in a nitrifying bioreactor that was used for treating saline high-strength ammonia wastewater. A PN system worked efficiently under saline conditions with varying operational factors, such as temperature, dissolved oxygen (DO), and alkalinity. Interestingly, the specific oxygen uptake rate (SOUR) became similar under salt-free and saline media after the salt adaption. Next generation sequencing results suggested that the inactivation of Nitrobacter winogradskyi was a key factor for the PN reaction under salt stress conditions. We also found that Nitrosomonas europaea, a freshwater type ammonia-oxidizing bacteria (AOB), was predominantly found under both salt-free and saline conditions, whereas other halotolerant or halophilic AOB species, including Nitrosomonas nitrosa and Nitrosomonas mobilis, became selectively abundant under saline conditions. This implies that adaptation (training of N. europaea) and selection (presence of N. nitrosa and N. mobilis) were simultaneously attributed to selective ammonia conversion for the PN reaction. The redundancy analysis showed that the salinity and ammonia loading rates were statistically significant process parameters that determined the nitrifying bacterial community, suggesting that these parameters drive the adaptation and selection of the core AOB species during the PN reaction. Furthermore, the correlation analysis revealed that the abundance of N. nitrosa and N. mobilis was critically correlated with the specific oxygen uptake rates in saline media containing ammonia. English Elsevier BV Improved insights into the adaptation and selection of Nitrosomonas spp. for partial nitritation under saline conditions based on specific oxygen uptake rates and next generation sequencing Article 10.1016/j.scitotenv.2022.153644 1 Science of the Total Environment, v.822 Science of the Total Environment 822 N scie scopus 000766801800008 2-s2.0-85124229179 Environmental Sciences Environmental Sciences & Ecology Article WASTE-WATER TREATMENT AMMONIA-OXIDIZING BACTERIA PARTIAL NITRIFICATION MICROBIAL COMMUNITY SP NOV. MEMBRANE BIOREACTOR OXIDATION-KINETICS LANDFILL LEACHATE ACTIVATED-SLUDGE LOADING RATE Partial nitritation Salinity Real-time quantitative PCR High-throughput sequencing Redundancy analysis