Lee Yeongseon Kwon, Osung Jeong, Geuk-Rae Noh, Junyeol Kim, Sung Eun Yi, Gwan-Su Hwang, Eun Mi Park, Jae-Yong 2024-01-19T12:04:26Z 2024-01-19T12:04:26Z 2022-05-04 2022-04 0024-3019 https://pubs.kist.re.kr/handle/201004/115307 The Tweety homolog (TTYH) chloride channel family is involved in oncogenic processes including cell proliferation, invasion, and colonization of cancers. Among the TTYH family, TTYH1 is highly expressed in several cancer cells, such as glioma, breast, and gastric cancer cells. However, the role of TTYH1 in the progression of osteosarcoma remains unknown. Here, we report that deficient TTYH1 expression results in the inhibition of the migration and invasion of U2OS human osteosarcoma cells. We found that TTYH1 was endogenously expressed at both mRNA and protein levels in U2OS cells and that these channels were located at the plasma membrane of the cells. Moreover, we found that silencing of the TTYH1 with small interfering RNA (siRNA) resulted in a decrease in the migration and invasion of U2OS cells, while the proliferation of the cells was not affected. Additionally, treatment with TTYH1 siRNA significantly suppressed the mRNA expression of epithelial-mesenchymal transition (EMT)-regulated transcription factors such as Zinc E-Box Binding Homeobox 1 (ZEB1) and SNAIL. Most importantly, the expression of matrix metalloproteinase (MMP)-2, MPP-9, and N-cadherin was dramatically reduced following the silencing of TTYH1. Taken together, our findings suggest that silencing of TTYH1 expression reduces migration and invasion of U2OS cells and that TTYH1 may act as a potential molecular target for osteosarcoma treatment. English Multidisciplinary Digital Publishing Institute (MDPI) Deficiency of TTYH1 Expression Reduces the Migration and Invasion of U2OS Human Osteosarcoma Cells Article 10.3390/life12040530 1 Life, v.12, no.4 Life 12 4 Y scie scopus 000785417300001 Biology Microbiology Life Sciences & Biomedicine - Other Topics Microbiology Article TO-EPITHELIAL TRANSITION IN-VITRO CANCER GENE PROGRESSION METASTASIS CHANNELS FAMILY SWITCH osteosarcoma TTYH1 invasion migration epithelial-mesenchymal transition