Park, Jun-Hee Lee, Ga-Yeon Song, Zhiquan Bong, Ji-Hong Chang, Young Wook Cho, Sungbo Kang, Min-Jung Pyun, Jae-Chul 2024-01-19T12:30:06Z 2024-01-19T12:30:06Z 2022-04-29 2022-04 0956-5663 https://pubs.kist.re.kr/handle/201004/115456 Vertically paired electrodes (VPEs) with multiple electrode pairs were developed for the enhancement of capacitive measurements by optimizing the electrode gap and number of electrode pairs. The electrode was fabricated using a conductive polymer layer of PEDOT:PSS instead of Ag and Pt metal electrodes to increase the VPE fabrication yield because the PEDOT:PSS layer could be effectively etched using a reactive dry etching process. In this study, sensitivity enhancement was realized by decreasing the electrode gap and increasing the number of VPE electrode pairs. Such an increase in sensitivity according to the electrode gap and the number of electrode pairs was estimated using a model analyte for an immunoassay. Additionally, a computer simulation was performed using VPEs with different electrode gaps and numbers of VPE electrode pairs. Finally, VPEs with multiple electrode pairs were applied for SARS-CoV-2 nucleoprotein (NP) detection. The capacitive biosensor based on the VPE with immobilized anti-SARS-CoV-2 NP was applied for the specific detection of SARS-CoV-2 in viral cultures. Using viral cultures of SARS-CoV-2, SARS-CoV, MERS-CoV, and CoV-strain 229E, the limit of detection (LOD) was estimated to satisfy the cutoff value (dilution factor of 1/800) for the medical diagnosis of COVID-19, and the assay results from the capacitive biosensor were compared with commercial rapid kit based on a lateral flow immunoassay. English Pergamon Press Ltd. Capacitive biosensor based on vertically paired electrodes for the detection of SARS-CoV-2 Article 10.1016/j.bios.2022.113975 1 BIOSENSORS & BIOELECTRONICS, v.202 BIOSENSORS & BIOELECTRONICS 202 N scie scopus 000782659200003 2-s2.0-85122749222 Biophysics Biotechnology & Applied Microbiology Chemistry, Analytical Electrochemistry Nanoscience & Nanotechnology Biophysics Biotechnology & Applied Microbiology Chemistry Electrochemistry Science & Technology - Other Topics Article INTERDIGITATED ELECTRODE TRANSFER KINETICS ESCHERICHIA-COLI SENSOR TEMPERATURE PERFORMANCE ANTIGEN IMMUNOASSAY DIAGNOSIS DAMAGE Capacitive biosensor SARS-CoV-2 Vertically paired electrode PEDOT PSS Nucleoprotein (NP) Immunoassay