Lee, Ju-Hyun Shin, Hyogeun Shaker, Mohammed R. Kim, Hyun Jung Park, Si-Hyung Kim, June Hoan Lee, Namwon Kang, Minjin Cho, Subin Kwak, Tae Hwan Kim, Jong Woon Song, Mi-Ryoung Kwon, Seung-Hae Han, Dong Wook Lee, Sanghyuk Choi, Se-Young Rhyu, Im Joo Kim, Hyun Geum, Dongho Cho, Il-Joo Sun, Woong 2024-01-19T12:30:16Z 2024-01-19T12:30:16Z 2022-04-14 2022-04 https://pubs.kist.re.kr/handle/201004/115464 Human spinal-cord-like tissues induced from human pluripotent stem cells are typically insufficiently mature and do not mimic the morphological features of neurulation. Here, we report a three-dimensional culture system and protocol for the production of human spinal-cord-like organoids (hSCOs) recapitulating the neurulation-like tube-forming morphogenesis of the early spinal cord. The hSCOs exhibited neurulation-like tube-forming morphogenesis, cellular differentiation into the major types of spinal-cord neurons as well as glial cells, and mature synaptic functional activities, among other features of the development of the spinal cord. We used the hSCOs to screen for antiepileptic drugs that can cause neural-tube defects. hSCOs may also facilitate the study of the development of the human spinal cord and the modelling of diseases associated with neural-tube defects. A protocol for the production of human spinal-cord-like organoids that recapitulate the tube-forming morphogenesis of the early human spinal cord facilitates screening for antiepileptic drugs that can cause neural-tube defects. English NATURE PUBLISHING GROUP Production of human spinal-cord organoids recapitulating neural-tube morphogenesis Article 10.1038/s41551-022-00868-4 1 Nature Biomedical Engineering, v.6, no.4, pp.435 - + Nature Biomedical Engineering 6 4 435 + N scie scopus 000773827900002 Engineering, Biomedical Engineering Article PLURIPOTENT STEM-CELLS BRAIN ORGANOIDS NEURULATION DYNAMICS RISK INTERNEURONS GENERATION DIVERSITY POLARITY PATTERN