Lee, Sang hee Jonghoon Kim Minseob Koh 2024-01-19T12:31:01Z 2024-01-19T12:31:01Z 2022-01-10 2022-04 0022-2836 https://pubs.kist.re.kr/handle/201004/115502 Technical innovations in protein labeling with a fluorophore at the specific residue have played a significant role in studying protein dynamics. The genetic code expansion (GCE) strategy enabled the precise installation of fluorophores at the tailored site of proteins in live cells with minimal perturbation of native functions. Considerable advances have been achieved over the past decades in fluorescent imaging using GCE strategies along with bioorthogonal chemistries. In this review, we discuss advances in the GCE-based strategies to site-specifically introduce fluorophore at a defined position of the protein and their bio-imaging applications. ？ 2021 Elsevier Ltd English Academic Press Recent Advances in Fluorescence Imaging by Genetically Encoded Non-canonical Amino Acids: Fluorescence imaging by genetically encoded ncAAs Article 10.1016/j.jmb.2021.167248 1 Journal of Molecular Biology, v.434, no.8, pp.167248 Journal of Molecular Biology 434 8 167248 N scie scopus 000817766600003 2-s2.0-85116140919 Biochemistry & Molecular Biology Biochemistry & Molecular Biology Review TRANSFER-RNA SYNTHETASE IN-VIVO INCORPORATION LEUCYL-TRANSFER-RNA CODE EXPANSION CLICK-CHEMISTRY BIOORTHOGONAL REACTIONS MAMMALIAN-CELLS PROTEIN PROBES DYNAMICS bio-imaging bioorthogonal chemistry fluorescence imaging genetic code expansion non-canonical amino acids