Park, Jung Hwan Jung, Euiyoung Lim, Hyeonji Lee, Ju-Ro Joung, Yoon Ki Yu, Taekyung Bhang, Suk Ho 2024-01-19T12:31:04Z 2024-01-19T12:31:04Z 2022-01-10 2022-04 1738-2696 https://pubs.kist.re.kr/handle/201004/115506 BACKGROUND: Owing to the tumor-targeted migration capacity of human mesenchymal stem cells (hMSCs), they have been combined with nanoparticles for photothermal therapy. However, the low viability of hMSCs following transplantation remains a problem. Here, we developed iron (Fe) ion-releasing gold (Au) nanoparticles (IIAuNPs) for advanced tumor-targeted photothermal therapy using hMSCs. METHODS: IIAuNPs were designed to undergo degradation under low pH conditions, such as the endosomal microenvironment, for Fe ion release in hMSCs. After evaluating the properties of IIAuNP, the IIAuNP concentration for treating hMSCs was optimized in terms of cytotoxicity. In vitro cell migration and antiapoptotic factor secretion were observed in hMSCs. Additionally, IIAuNPs-treated hMSCs were intravenously injected into tumor-bearing mice, and enhanced tumor targeting based on improved cell viability and cell migration was evaluated. Three days after the injection, the mice were irradiated with 660 nm laser to confirm the enhanced photothermal effect. RESULTS: In vitro studies revealed that treating hMSCs with an optimum concentration of IIAuNPs enhanced cell migration and anti-apoptotic gene expression through intracellular Fe ion delivery. The viability of hMSCs under hypoxic cell culture conditions that mimic the in vivo microenvironment was also improved when hMSCs were treated with IIAuNPs, compared to hMSCs without IIAuNPs treatment. IIAuNPs-treated hMSCs showed significantly enhanced tumor-targeting efficiency and subsequent photothermal effect compared to hMSCs without IIAuNP treatment. CONCLUSION: Our results suggest that our metal-ion-releasing photothermal nanoparticles may provide a promising platform for future photothermal therapies and related applications. English KOREAN TISSUE ENGINEERING REGENERATIVE MEDICINE SOC Metal Ion Releasing Gold Nanoparticles for Improving Therapeutic Efficiency of Tumor Targeted Photothermal Therapy Article 10.1007/s13770-021-00385-6 1 TISSUE ENGINEERING AND REGENERATIVE MEDICINE, v.19, no.2, pp.289 - 299 TISSUE ENGINEERING AND REGENERATIVE MEDICINE 19 2 289 299 N scie scopus kci ART002832147 000698864100001 2-s2.0-85115607812 Cell & Tissue Engineering Engineering, Biomedical Cell Biology Engineering Article; Early Access MESENCHYMAL STEM-CELLS IN-VITRO MIGRATION EFFICACY Human mesenchymal stem cell Metal ion delivery Photothermal therapy