Song, Eui Sun Park, Joo-Hee Ha, Sang Su Cha, Pu-hyeon Kang, Jung-Taek Park, Choul Yong Park, Kwideok 2024-01-19T12:31:25Z 2024-01-19T12:31:25Z 2022-05-24 2022-03 1944-8244 https://pubs.kist.re.kr/handle/201004/115526 Here, we report a transparent, biodegradable, and cell-adhesive carrier that is securely coupled with the extracellular matrix (ECM) for corneal endothelial cell (CEC) transplantation. To fabricate a CEC carrier, poly(lactide-co-caprolactone) (PLCL) solution was poured onto the decellularized ECM (UMDM) derived from in vitro cultured umbilical cord blood-MSCs. Once completely dried, ECM-PLCL was then peeled off from the substrate. It was 20 mu m thick, transparent, rich in fibronectin and collagen type IV, and easy to handle. Surface characterizations exhibited that ECM-PLCL was very rough (54.0 +/- 4.50 nm) and uniformly covered in high density by ECM and retained a positive surface charge (65.2 +/- 57.8 mV), as assessed via atomic force microscopy. Human CECs (B4G12) on the ECM-PLCL showed good cell attachment, with a cell density similar to the normal cornea. They could also maintain a cell phenotype, with nicely formed cell-cell junctions as assessed via ZO-1 and N-cadherin at 14 days. This was in sharp contrast to the CEC behaviors on the FNC-coated PLCL (positive control). A function-related marker, Na+/K+-ATPase, was also identified via western blot and immunofluorescence. In addition, primary rabbit CECs showed a normal shape and they could express structural and functional proteins on the ECM-PLCL. A simulation test confirmed that CECs loaded on the ECM-PLCL were successfully engrafted into the decellularized porcine corneal tissue, with a high engraftment level and cell viability. Moreover, ECM-PLCL transplantation into the anterior chamber of the rabbit eye for 8 weeks proved the maintenance of normal cornea properties. Taken together, this study demonstrates that our ECM-PLCL can be a promising cornea endothelium graft with an excellent ECM microenvironment for CECs. English American Chemical Society Novel Corneal Endothelial Cell Carrier Couples a Biodegradable Polymer and a Mesenchymal Stem Cell-Derived Extracellular Matrix Article 10.1021/acsami.2c01709 1 ACS Applied Materials & Interfaces, v.14, no.10, pp.12116 - 12129 ACS Applied Materials & Interfaces 14 10 12116 12129 N scie scopus 000787549000009 Nanoscience & Nanotechnology Materials Science, Multidisciplinary Science & Technology - Other Topics Materials Science Article HEPATOCYTE GROWTH-FACTOR HYDROGEL FILMS REGENERATION DEGRADATION SCAFFOLDS cornea corneal endothelial cell (CEC) decellularized extracellular matrix poly(lactide-co-caprolactone) (PLCL) CEC carrier