Rajalakshmi, Kanagaraj Deng, Tongtong Muthusamy, Selvaraj Xie, Meng Xie, Jimin Lee, Kang-Bong Xu, Yuanguo 2024-01-19T12:32:26Z 2024-01-19T12:32:26Z 2022-04-03 2022-03 1386-1425 https://pubs.kist.re.kr/handle/201004/115585 Citrate is a tricarboxylate, plays vital role in prostate cancer (PC) and the level of citrate is an indicator for PC identification. Herein, triaminoguanidine carbon dots (TAG-CDs) prepared by one step hydrothermal method and used as a citrate receptor. Notably the TAG-CDs without alkaline treatment were highly fluorescent at pH 7 with high quantum yield (11.3%). TAG-CDs were characterized through TEM, XRD, FT-IR, UV-vis and spectrofluorimetry. It is noted that the average size was of 2.8 nm, the presence of highly disordered carbon, retain the functionality of TAG. The absorbance maxima obtained at 294 nm and good emitting response observed at 396 nm. The Y-aromaticity of receptor guanidinium moiety acts as Lewis acid and have peculiar interaction with Lewis base citrate via electrostatic interaction and also protons in the TAG participate hydrogen bonds with citrate, which causes quenching of TAG-CDs. From the obtained linear quenching equation the LOD was found to be 4 nM. The probe expressed high selectivity, high interference tolerance (500 - fold), fast response in 15 mins and good biocompatible. Finally, TAG-CDs utilized for the intracellular imaging of citrate in live MCF-7 cells, it showed good cytotoxicity and delivered contrast images in presence, absence of citrate. TAG-CDs detected the citrate level in human urine samples, the obtained results are validated with HPLC method. (C) 2021 Elsevier B.V. All rights reserved. English Pergamon Press Ltd. Prostate cancer biomarker citrate detection using triaminoguanidinium carbon dots, its applications in live cells and human urine samples Article 10.1016/j.saa.2021.120622 1 Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, v.268 Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 268 N scie scopus 000753652800017 2-s2.0-85120734572 Spectroscopy Spectroscopy Article FLUORESCENT INDICATOR GREEN SYNTHESIS SENSOR COMPLEX NANOPARTICLES RECOGNITION CHEMOSENSOR ACID Lewis acid-base sensor Carbon dots Triaminoguanidinium receptor Prostate cancer biomarker Fluorescent citrate sensor Bioimaging in MCF-7 cells