Lim, Wonchul Jeon, Bu-Nam Kim, Young-Joo Kim, Ki-Hwan Ko, Hyeonseok 2024-01-19T12:32:55Z 2024-01-19T12:32:55Z 2022-01-25 2022-03 0730-2312 https://pubs.kist.re.kr/handle/201004/115610 The factor binding inducer of short transcripts-1 (FBI-1) is a POZ-domain Kruppel-like (POK) family of transcription factors and is known as a protooncogene or tumor suppressor in various carcinomas. However, the role of FBI-1 on epithelial-to-mesenchymal transition (EMT) and invasiveness in lung cancer remains unknown. Preliminarily, clinical data such as tissue microarray, Kaplan-Meier, and Oncomine were analyzed to confirm the correlation between lung cancer metastasis and FBI-1. To investigate the function of FBI-1 in EMT in lung cancer, EMT was measured in FBI-1-deficient or FBI-1-overexpressing cells. FBI-1 showed decreased expression in tumors metastasized to lymph nodes than in the primary tumor. In addition, it was also associated with improved survival rates of lung cancer patients. FBI-1 knockdown improved E-to-N-cadherin switching, migration, and invasion in A549 cells, similar to the initiation of EMT stimulated by transforming growth factor- beta 1 (TGF-beta 1). In contrast, overexpression of FBI-1 inhibited the transcription and activation of Smad2, thereby interfering with EMT, despite stimulation by TGF-beta 1. These results suggest that FBI-1 plays a negative role in EMT in lung cancer via the TGF-beta 1 signaling pathway, implying its use as a new potential therapeutic target and diagnostic indicator for early stage of lung cancer metastasis. English WILEY FBI-1 inhibits epithelial-to-mesenchymal transition, migration, and invasion in lung adenocarcinoma A549 cells by downregulating transforming growth factor-beta 1 signaling pathway Article 10.1002/jcb.30210 1 JOURNAL OF CELLULAR BIOCHEMISTRY, v.123, no.3, pp.644 - 656 JOURNAL OF CELLULAR BIOCHEMISTRY 123 3 644 656 N scie scopus 000739097100001 2-s2.0-85122322474 Biochemistry & Molecular Biology Cell Biology Biochemistry & Molecular Biology Cell Biology Article; Early Access METASTASIS epithelial-to-mesenchymal transition (EMT) FBI-1 lung cancer transforming growth factor-beta 1 (TGF-beta 1)