Kim, Hyosuk Song, Byeong-Wook Park, Soon-Jung Choi, Seong Woo Moon, Hanbyeol Hwang, Ki-Chul Kang, Sun-Woong Moon, Sung-Hwan Yang, Yoosoo Kwon, Ick Chan Kim, Sun Hwa 2024-01-19T12:33:54Z 2024-01-19T12:33:54Z 2022-04-05 2022-02 2375-2548 https://pubs.kist.re.kr/handle/201004/115669 Direct lineage conversion holds great promise in the regenerative medicine field for restoring damaged tissues using functionally engineered counterparts. However, current methods of direct lineage conversion, even those using virus-mediated transgenic expression of tumorigenic factors, are extremely inefficient (similar to 25%). Thus, advanced methodologies capable of revolutionizing efficiency and addressing safety concerns are key to clinical translation of these technologies. Here, we propose an extracellular vesicle (EV)-guided, nonviral, direct lineage conversion strategy to enhance transdifferentiation of fibroblasts to induced cardiomyocyte-like cells (iCMs). The resulting iCMs have typical cardiac Ca2+ transients and electrophysiological features and exhibit global gene expression profiles similar to those of cardiomyocytes. This is the first demonstration of the use of EVs derived from embryonic stem cells undergoing cardiac differentiation as biomimetic tools to induce cardiac reprogramming with extremely high efficiency (>60%), establishing a general, more readily accessible platform for generating a variety of specialized somatic cells through direct lineage conversion. English American Association for the Advancement of Science Ultraefficient extracellular vesicle-guided direct reprogramming of fibroblasts into functional cardiomyocytes Article 10.1126/sciadv.abj6621 1 Science Advances, v.8, no.8 Science Advances 8 8 Y scie scopus 000763313300006 2-s2.0-85125354377 Multidisciplinary Sciences Science & Technology - Other Topics Article PLURIPOTENT STEM-CELLS CARDIAC DIFFERENTIATION MOUSE FIBROBLASTS SOMATIC-CELLS MECHANICAL LOSS EXOSOMES PROTEIN PROGRESS HEART