Choi, Woong sun Park, Eunhye Bae, Seojin Choi, Kyung-Hak Han, Sangeun Son, Kuk-Hui Lee, Do Young CHO, IL JOO Seong, Hye jeong Hwang, Kyo Seon Nam, Jwa-Min Choi, Jungkyu LEE, HYO JIN Choi, Nakwon 2024-01-19T13:00:44Z 2024-01-19T13:00:44Z 2022-01-10 2022-02 1613-6810 https://pubs.kist.re.kr/handle/201004/115791 Single nucleotide polymorphisms (SNPs) that can alter phenotypes of individuals play a pivotal role in disease development and, more importantly, responses to therapy. However, SNP genotyping has been challenging due to the similarity of SNP alleles and their low concentration in biological samples. Sequence-specific nanoparticle with interpretative toehold-mediated sequence decoding in hydrogel (SWITCH) for multiplex SNP genotyping is presented. The encoding with gold nanoparticle probes transduces each SNP target to ？1000 invaders with prominently different sequences between wild and mutant types, featuring polymerase chain reaction (PCR)-free amplification. Subsequently, the toehold-mediated DNA replacement in hydrogel microparticles decodes the invaders via SNP-specific fluorescence signals. The 4-plex detection of the warfarin-associated SNP targets spiked in commercially validated human serum (S1-100ML, Merck) is successfully demonstrated with excellent specificity. This work is the first technology development presenting PCR-free, multiplex SNP genotyping with a single reporting fluorophore, to the best of knowledge. ？ 2021 The Authors. Small published by Wiley-VCH GmbH English Wiley - V C H Verlag GmbbH & Co. Multiplex SNP Genotyping Using SWITCH: Sequence-Specific Nanoparticle with Interpretative Toehold-Mediated Sequence Decoding in Hydrogel Article 10.1002/smll.202105538 1 Small, v.18, no.8 Small 18 8 Y scie scopus 000731409800001 2-s2.0-85121448497 Chemistry, Multidisciplinary Chemistry, Physical Nanoscience & Nanotechnology Materials Science, Multidisciplinary Physics, Applied Physics, Condensed Matter Chemistry Science & Technology - Other Topics Materials Science Physics Article ALLELE-SPECIFIC PCR DNA PURIFICATION STRAND-DISPLACEMENT POLYMORPHISMS DISCOVERY VKORC1 MICROPARTICLES IDENTIFICATION ASSOCIATION GENETICS hydrogel microparticles magnetic microparticles multiplex single nucleotide polymorphism genotyping sandwich assays toehold-mediated DNA displacement warfarin gold nanoparticles