Wang, Yunxuan Meng, Xianzhi Tian, Yang Kim, Kwang Ho Jia, Linjing Pu, Yunqiao Leem, Gyu Kumar, Deepak Eudes, Aymerick Ragauskas, Arthur J. Yoo, Chang Geun 2024-01-19T13:03:12Z 2024-01-19T13:03:12Z 2022-01-10 2021-12-06 1864-5631 https://pubs.kist.re.kr/handle/201004/115942 Integrating multidisciplinary research in plant genetic engineering and renewable deep eutectic solvents (DESs) can facilitate a sustainable and economic biorefinery. Herein, we leveraged a plant genetic engineering approach to specifically incorporate C6C1 monomers into the lignin structure. By expressing the bacterial ubiC gene in sorghum, p-hydroxybenzoic acid (PB)-rich lignin was incorporated into the plant cell wall while this monomer was completely absent in the lignin of the wild-type (WT) biomass. A DES was synthesized with choline chloride (ChCl) and PB and applied to the pretreatment of the PB-rich mutant biomass for a sustainable biorefinery. The release of fermentable sugars was significantly enhanced (similar to 190 % increase) compared to untreated biomass by the DES pretreatment. In particular, the glucose released from the pretreated mutant biomass was up to 12 % higher than that from the pretreated WT biomass. Lignin was effectively removed from the biomass with the preservation of more than half of the beta-omicron-4 linkages without condensed aromatic structures. Hydrogenolysis of the fractionated lignin was conducted to demonstrate the potential of phenolic compound production. In addition, a simple hydrothermal treatment could selectively extract PB from the same engineered lignin, showing a possible circular biorefinery. These results suggest that the combination of PB-based DES and engineered PB-rich biomass is a promising strategy to achieve a sustainable closed-loop biorefinery. English WILEY-V C H VERLAG GMBH TEMPERATURE MIXTURES LTTMS BIOMASS RECALCITRANCE ENZYMATIC-HYDROLYSIS GREEN SOLVENTS RICE STRAW LIGNIN PRETREATMENT FRACTIONATION TRANSITION CELLULOSE Engineered Sorghum Bagasse Enables a Sustainable Biorefinery with p-Hydroxybenzoic Acid-Based Deep Eutectic Solvent Article 10.1002/cssc.202101492 1 CHEMSUSCHEM, v.14, no.23, pp.5235 - 5244 CHEMSUSCHEM 14 23 5235 5244 scie scopus 000708102500001 2-s2.0-85117218467 Chemistry, Multidisciplinary Green & Sustainable Science & Technology Chemistry Science & Technology - Other Topics Article TEMPERATURE MIXTURES LTTMS BIOMASS RECALCITRANCE ENZYMATIC-HYDROLYSIS GREEN SOLVENTS RICE STRAW LIGNIN PRETREATMENT FRACTIONATION TRANSITION CELLULOSE biorefinery green solvent lignin lignin depolymerization sustainable process