Ha, Sang Su Kim, Jung-Hyun Savitri, Cininta Choi, Donghoon Park, Kwideok 2024-01-19T13:03:31Z 2024-01-19T13:03:31Z 2022-04-03 2021-12 1661-6596 https://pubs.kist.re.kr/handle/201004/115959 Cell-derived matrix (CDM) has proven its therapeutic potential and been utilized as a promising resource in tissue regeneration. In this study, we prepared a human fibroblast-derived matrix (FDM) by decellularization of in vitro cultured cells and transformed the FDM into a nano-sized suspended formulation (sFDM) using ultrasonication. The sFDM was then homogeneously mixed with Pluronic F127 and hyaluronic acid (HA), to effectively administer sFDM into target sites. Both sFDM and sFDM containing hydrogel (PH/sFDM) were characterized via immunofluorescence, sol-gel transition, rheological analysis, and biochemical factors array. We found that PH/sFDM hydrogel has biocompatible, mechanically stable, injectable properties and can be easily administered into the external and internal target regions. sFDM itself holds diverse bioactive molecules. Interestingly, sFDM-containing serum-free media helped maintain the metabolic activity of endothelial cells significantly better than those in serum-free condition. PH/sFDM also promoted vascular endothelial growth factor (VEGF) secretion from monocytes in vitro. Moreover, when we evaluated therapeutic effects of PH/sFDM via the murine full-thickness skin wound model, regenerative potential of PH/sFDM was supported by epidermal thickness, significantly more neovessel formation, and enhanced mature collagen deposition. The hindlimb ischemia model also found some therapeutic improvements, as assessed by accelerated blood reperfusion and substantially diminished necrosis and fibrosis in the gastrocnemius and tibialis muscles. Together, based on sFDM holding a strong therapeutic potential, our engineered hydrogel (PH/sFDM) should be a promising candidate in tissue engineering and regenerative medicine. English MDPI Nano-Sized Extracellular Matrix Particles Lead to Therapeutic Improvement for Cutaneous Wound and Hindlimb Ischemia Article 10.3390/ijms222413265 1 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v.22, no.24 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 22 24 Y scie scopus 000738600100001 2-s2.0-85120773306 Biochemistry & Molecular Biology Chemistry, Multidisciplinary Biochemistry & Molecular Biology Chemistry Article HYALURONIC-ACID ANGIOGENESIS HYDROGEL DIFFERENTIATION MECHANISMS SCAFFOLDS COLLAGEN GROWTH fibroblast-derived matrix (FDM) decellularization suspended FDM Pluronic F127 hyaluronic acid wound healing hindlimb ischemia