Jung, Yoonji Artan, Murat Kim, Nari Yeom, Jeonghun Hwang, Ara B. Jeong, Dae-Eun Altintas, Ozlem Seo, Keunhee Seo, Mihwa Lee, Dongyeop Hwang, Wooseon Lee, Yujin Sohn, Jooyeon Kim, Eun Ji E. Ju, Sungeun Han, Seong Kyu Nam, Hyun-Jun Adams, Linnea Ryu, Youngjae Moon, Dong Jin Kang, Chanhee Yoo, Joo-Yeon Park, Sang Ki Ha, Chang Man Hansen, Malene Kim, Sanguk Lee, Cheolju Park, Seung-Yeol Lee, Seung-Jae, V 2024-01-19T13:03:58Z 2024-01-19T13:03:58Z 2022-01-25 2021-12 2375-2548 https://pubs.kist.re.kr/handle/201004/115985 The Golgi apparatus plays a central role in trafficking cargoes such as proteins and lipids. Defects in the Golgi apparatus lead to various diseases, but its role in organismal longevity is largely unknown. Using a quantitative proteomic approach, we found that a Golgi protein, MON-2, was up-regulated in long-lived Caenorhabditis elegans mutants with mitochondrial respiration defects and was required for their longevity. Similarly, we showed that DOP1/PAD-1, which acts with MON-2 to traffic macromolecules between the Golgi and endosome, contributed to the longevity of respiration mutants. Furthermore, we demonstrated that MON-2 was required for up-regulation of autophagy, a longevity-associated recycling process, by activating the Atg8 ortholog GABARAP/LGG-1 in C. elegans. Consistently, we showed that mammalian MON2 activated GABARAPL2 through physical interaction, which increased autophagic flux in mammalian cells. Thus, the evolutionarily conserved role of MON2 in trafficking between the Golgi and endosome is an integral part of autophagy-mediated longevity. English AMER ASSOC ADVANCEMENT SCIENCE LIFE-SPAN EXCHANGE FACTORS TRANSPORT ROS MITOCHONDRIA ENDOCYTOSIS MECHANISMS INTERACTS GABARAP PATHWAY MON-2, a Golgi protein, mediates autophagy-dependent longevity in Caenorhabditis elegans Article 10.1126/sciadv.abj8156 1 SCIENCE ADVANCES, v.7, no.49 SCIENCE ADVANCES 7 49 scie scopus 000730595900006 2-s2.0-85120666559 Multidisciplinary Sciences Science & Technology - Other Topics Article LIFE-SPAN EXCHANGE FACTORS TRANSPORT ROS MITOCHONDRIA ENDOCYTOSIS MECHANISMS INTERACTS GABARAP PATHWAY