Kim, Kyoung-Ran Kim, Junghyun Mao, Chengde Ahn, Dae-Ro 2024-01-19T13:03:58Z 2024-01-19T13:03:58Z 2022-01-25 2021-12 2047-4830 https://pubs.kist.re.kr/handle/201004/115986 We describe an efficient method to condense RNAs into tightly packed RNA nanoparticles (RNPs) for biomedical applications without hydrophobic or cationic agents. We embedded kissing loops and siRNA in the RNAs to constrain the size of RNPs to ca. 100 nm, making them suitable not only for cellular uptake but also for passive tumor accumulation. The resulting RNPs were efficiently internalized into cells and downregulated the target gene of siRNAs. When intravenously injected into tumor-bearing mice, RNPs could also accumulate in the tumor. The reported fabrication method could be readily adopted as a platform to prepare RNPs for in vitro and in vivo delivery of bioactive RNAs. English Royal Society of Chemistry Kissing loop-mediated fabrication of RNA nanoparticles and their potential as cellular and in vivo siRNA delivery platforms Article 10.1039/d1bm01440d 1 Biomaterials Science, v.9, no.24, pp.8148 - 8152 Biomaterials Science 9 24 8148 8152 N scie scopus 000716557200001 2-s2.0-85121046635 Materials Science, Biomaterials Materials Science Article ROLLING CIRCLE TRANSCRIPTION CIRCULAR OLIGONUCLEOTIDES SIZE AMPLIFICATION INTERFERENCE PARTICLES