An, Jusung Jangili, Paramesh Lim, Sungsu Kim, Yun Kyung Verwilst, Peter Kim, Jong Seung 2024-01-19T13:04:20Z 2024-01-19T13:04:20Z 2022-01-10 2021-12 1388-3127 https://pubs.kist.re.kr/handle/201004/116008 The pathological origin of Alzheimer’s disease (AD) remains uncharted terrain, despite intensive investigation. Discriminating aberrant proteinaceous deposits, such as β-amyloid (Aβ) and (hyperphosphorylated) tau protein by imaging methods, is a vital tool to support investigations towards the network of interacting features that results in AD pathology. In this context, multispectral fluorescence imaging (MSFI) has drawn much attention enabling the distinction of several analytes with merely a single fluorophore emitting a multichromatic fluorescent signal. Herein, we developed three kinds of benzimidazole-derived fluorescent dyes, BZ1–BZ3. The photophysical properties and intramolecular charge transfer (ICT) characteristics of the probes were evaluated in various solvents. Furthermore, a benzimidazole-associated polar-sensitivity displayed multichromatic behavior and enabled the visualization of minute differences in microenvironmental polarity between Aβ and tau aggregates, resulting in different maximal fluorescent emission wavelengths. Indeed, BZ2 demonstrated an approximately 30 nm bathochromic shift in maximal fluorescent emission. All these observations offer a potential for the development of a future generation of benzimidazole-derived ICT-based fluorescent probes. English Kluwer Academic Publishers Multichromatic fluorescence towards aberrant proteinaceous aggregates utilizing benzimidazole-based ICT fluorophores Article 10.1007/s10847-021-01085-3 1 Journal of Inclusion Phenomena, v.101, pp.205 - 215 Journal of Inclusion Phenomena 101 205 215 N scie scopus 000658093400001 2-s2.0-85110382138 Chemistry, Multidisciplinary Chemistry Article; Early Access THIOPHENE-BASED LIGANDS IN-VITRO EVALUATION AMYLOID-BETA ALZHEIMERS-DISEASE TAU PATHOLOGY NEUROFIBRILLARY TANGLES BIOLOGICAL EVALUATION CROSS-LINKING DESIGN PROBES Multispectral fluorescence imaging Benzimidazole-derivatives Intramolecular charge transfer Microenvironmental polarity Alzheimer&apos s disease