Kim, Junbeom Shim, Ji Sung Han, Bo Hoon Kim, Hye Jin Park, Jaesung Cho, Il-Joo Kang, Sung Gu Kang, Ji Yoon Bong, Ki Wan Choi, Nakwon 2024-01-19T13:30:43Z 2024-01-19T13:30:43Z 2021-10-21 2021-11-15 0956-5663 https://pubs.kist.re.kr/handle/201004/116132 Although urinary exosomal microRNAs (miRNAs) have recently emerged as potential biomarkers, clinical applications are still limited due to their low concentration in small volumes of clinical samples. Therefore, the development of a non-invasive, specific diagnostic tool, along with profiling exosomal miRNA markers from urine, remains a significant challenge. Here, we present hydrogel-based hybridization chain reaction (HCR) for multiplex signal amplification to detect urinary exosomal miRNAs from human clinical samples. We succeeded in identifying small amounts (similar to amol) of exosomal miRNAs from 600 mu L of urine with up to similar to 35-fold amplification and enhanced detection limits by over an order of magnitude for two miRNA biomarker candidates, hsa-miR6090 and hsa-miR-3665. Furthermore, we proposed ratiometric analysis without requiring normalization to a reference miRNA and validated the clinical diagnostic potential toward differentiating prostate cancer patients from healthy controls. Our hydrogel-based HCR could serve as a new diagnostic platform for a non-invasive liquid biopsy before burdensome tissue biopsy of various diseases, including prostate cancer screening, complementing the PSA test. English ELSEVIER ADVANCED TECHNOLOGY LIQUID BIOPSY MULTIPLEXED DETECTION MICRORNA DETECTION POTENTIAL BIOMARKERS MICROPARTICLES EXPRESSION AMPLIFICATION PROGRESSION PARTICLES PLATFORM Hydrogel-based hybridization chain reaction (HCR) for detection of urinary exosomal miRNAs as a diagnostic tool of prostate cancer Article 10.1016/j.bios.2021.113504 1 BIOSENSORS & BIOELECTRONICS, v.192 BIOSENSORS & BIOELECTRONICS 192 scie scopus 000703541600004 2-s2.0-85110556830 Biophysics Biotechnology & Applied Microbiology Chemistry, Analytical Electrochemistry Nanoscience & Nanotechnology Biophysics Biotechnology & Applied Microbiology Chemistry Electrochemistry Science & Technology - Other Topics Article LIQUID BIOPSY MULTIPLEXED DETECTION MICRORNA DETECTION POTENTIAL BIOMARKERS MICROPARTICLES EXPRESSION AMPLIFICATION PROGRESSION PARTICLES PLATFORM Urinary exosome miRNA Hydrogel microparticle Diagnostics Prostate cancer