Rhee, Jun Kyu Iwamoto, Yayoi Baker, Bradley J. 2024-01-19T13:30:57Z 2024-01-19T13:30:57Z 2022-01-10 2021-11-02 1662-5129 https://pubs.kist.re.kr/handle/201004/116146 Genetically encoded voltage indicators (GEVIs) expressed pan-neuronally were able to optically resolve bicuculline induced spontaneous oscillations in brain slices of the mouse motor cortex. Three GEVIs were used that differ in their timing of response to voltage transients as well as in their voltage ranges. The duration, number of cycles, and frequency of the recorded oscillations reflected the characteristics of each GEVI used. Multiple oscillations imaged in the same slice never originated at the same location, indicating the lack of a "hot spot" for induction of the voltage changes. Comparison of pan-neuronal, Ca2+/calmodulin-dependent protein kinase II alpha restricted, and parvalbumin restricted GEVI expression revealed distinct profiles for the excitatory and inhibitory cells in the spontaneous oscillations of the motor cortex. Resolving voltage fluctuations across space, time, and cell types with GEVIs represent a powerful approach to dissecting neuronal circuit activity. English FRONTIERS MEDIA SA HIGH-FREQUENCY STIMULATION PRIMARY MOTOR CORTEX INTRACELLULAR-RECORDINGS GABAERGIC INTERNEURONS MOVEMENT-DISORDERS NEURONS ADULT PARVALBUMIN NEOCORTEX NETWORKS Visualizing Oscillations in Brain Slices With Genetically Encoded Voltage Indicators Article 10.3389/fnana.2021.741711 1 FRONTIERS IN NEUROANATOMY, v.15 FRONTIERS IN NEUROANATOMY 15 scie scopus 000719650500001 2-s2.0-85119263367 Anatomy & Morphology Neurosciences Anatomy & Morphology Neurosciences & Neurology Article HIGH-FREQUENCY STIMULATION PRIMARY MOTOR CORTEX INTRACELLULAR-RECORDINGS GABAERGIC INTERNEURONS MOVEMENT-DISORDERS NEURONS ADULT PARVALBUMIN NEOCORTEX NETWORKS motor cortex neural population activity oscillations genetically encoded voltage indicators ArcLight Bongwoori-Pos6 Bongwoori-R3