Khan, Abdul Latif Asaf, Sajjad Numan, Muhammad AbdulKareem, Noor Mazin Imran, Muhammad Riethoven, Jean-Jack M. Kim, Ho-Youn Al-Harrasi, Ahmed Schachtman, Daniel P. Al-Rawahi, Ahmed Lee, In-Jung 2024-01-19T13:31:14Z 2024-01-19T13:31:14Z 2022-04-03 2021-11 0888-7543 https://pubs.kist.re.kr/handle/201004/116163 Frankincense tree (Boswellia sacra Fluek) has been poorly known on how it responds to tapping and woundrecovery process at molecular levels. Here, we used RNA-sequencing analysis to profile transcriptome of B. sacra after 30 min, 3 h and 6 h of post-tapping. Results showed 5525 differentially expressed genes (DEGs) that were related to terpenoid biosynthesis, phytohormonal regulation, cellular transport, and cell-wall synthesis. Plant-growth-regulators were applied exogenously which showed regulation of endogenous jasmonates and resulted in rapid recovery of cell-wall integrity by significantly up-regulated gene expression of terpenoid biosynthesis (germacrene-D synthase, B-amyrin synthase, and squalene epioxidase-1) and cell-wall synthesis (xyloglucan endotransglucosylase, cellulose synthase-A, and cell-wall hydrolase) compared to control. These findings suggest that tapping immediately activated several cell-developmental and regeneration processes, alongwith defense-induced terpenoid metabolism, to improve the healing process in epidermis. Exogenous growth regulators, especially jasmonic acid, can drastically help tree recovery from tissue degeneration and might help in tree conservation purposes. English ACADEMIC PRESS INC ELSEVIER SCIENCE SALICYLIC-ACID JASMONIC ACID PLANT DEFENSE STRESS EXPRESSION BIOSYNTHESIS SYNTHASES TOMATO AUXIN TIME Transcriptomics of tapping and healing process in frankincense tree during resin production Article 10.1016/j.ygeno.2021.11.020 1 GENOMICS, v.113, no.6, pp.4337 - 4351 GENOMICS 113 6 4337 4351 scie scopus 000730898100002 2-s2.0-85119604978 Biotechnology & Applied Microbiology Genetics & Heredity Biotechnology & Applied Microbiology Genetics & Heredity Article SALICYLIC-ACID JASMONIC ACID PLANT DEFENSE STRESS EXPRESSION BIOSYNTHESIS SYNTHASES TOMATO AUXIN TIME Boswellia sacra Ecophysiolomics Gene expression Phytohormones Plant-growth regulators Wounding stress