Ha, Geon-Soo Saha, Shouvik Basak, Bikram Kurade, Mayur B. Kim, Gyeong-Uk Ji, Min-Kyu Ahn, Yongtae Salama, El-Sayed Chang, Soon Woong Jeon, Byong-Hun 2024-01-19T13:31:22Z 2024-01-19T13:31:22Z 2022-01-25 2021-11 0960-8524 https://pubs.kist.re.kr/handle/201004/116172 The commercial feasibility of energy-efficient conversion of highly concentrated microalgal suspensions to produce high-titer biofuels is a major bottleneck due to high energy consumption. Herein, high-titer biofuels (bioethanol, higher-alcohols, and biodiesel) were generated from carbohydrate-rich Chlamydomonas mexicana and lipid-rich Chlamydomonas pitschmannii biomass through energy-saving microwave pretreatment, successive fermentation, and transesterification. Microwave pretreatment needed low specific energy (4.2 MJ/kg) for 100 g/L of microalgal suspension. Proposed sustainable integrated pretreatments method achieved unprecedented total conversion efficiency (67%) and highest biomass utilization (87%) of C. pitschmannii (100 g/L) with high yields of bioethanol (0.48 g-ethanol/g-carbohydrates), higher-alcohols (0.44 g-higher-alcohols/g-proteins), and biodiesel (0.90 g-biodiesel/g-lipids). Transmission electron microscopy showed the changes in the microalgal cellular integrity before and after sequential fermentations. Energy-efficient integrated pretreatments enhanced the extraction efficiency and whole utilization of high-concentration microalgae to generate high-titer biofuels with minimum waste production. English ELSEVIER SCI LTD High-throughput integrated pretreatment strategies to convert high-solid loading microalgae into high-concentration biofuels Article 10.1016/j.biortech.2021.125651 1 BIORESOURCE TECHNOLOGY, v.340 BIORESOURCE TECHNOLOGY 340 N scie scopus 000697026100013 2-s2.0-85111341738 Agricultural Engineering Biotechnology & Applied Microbiology Energy & Fuels Agriculture Biotechnology & Applied Microbiology Energy & Fuels Article BIODIESEL PRODUCTION BIOMASS MICROWAVE LIPIDS BIOREFINERY EXTRACTION BIOETHANOL TRANSESTERIFICATION BIOENERGY Microalgal biorefinery High titer biofuel Integrated pretreatment Waste management Cell disruption