Noh, Joo-Yoon Kim, Moon-Ju Kim, Mira Kim, Jo-Il Park, Jong-Min Yun, Tae Gyeong Kang, Min-Jung Pyun, Jae-Chul 2024-01-19T13:31:28Z 2024-01-19T13:31:28Z 2022-01-25 2021-11 2093-3134 https://pubs.kist.re.kr/handle/201004/116178 A novel method for quantifying galactose was developed to serve as a newborn screening test for galactosemia using laser desorption/ionization time-of-flight (LDI-TOF) mass spectrometry (MS) with a TiO2 nanowire chip. Herein, phosphate citrate buffer, serum, and dried blood spot (DBS) were employed for the quantitative analysis of galactose. To quantitatively analyze galactose, its reduction potential was used to oxidize o-phenylene diamine (OPD) into 2,3-diaminophenazine (DA), which were both detected using LDI-TOF MS with a TiO2 nanowire chip according to the concentration of galactose. The reproducibility and the interference of glucose were determined to demonstrate the applicability of this method. Moreover, mixtures of galactose, phenylalanine, and 17 alpha-OHP were analyzed to determine the interference induced by other biomarkers of metabolic disorders. The OPD oxidation of galactose was found to be selectively achieved under high-glucose conditions, similar to human blood, thereby showing good reproducibility. The intensities of the mass peaks of OPD and DA based on LDI-TOF MS with a TiO2 nanowire chip were linearly correlated in the galactose concentration range of 57.2-220.0 mu g/mL (r(2) = 0.999 and 0.950, respectively) for serum samples and 52.5-220.0 mu g/mL (r(2) = 0.993 and 0.985, respectively) for DBS after methanol precipitation/extraction. The enzyme immunoassay and LDI-TOF MS analysis results were statistically analyzed, and a mixture of phenylalanine, 17 alpha-OHP, and galactose was simultaneously investigated quantitatively at the cutoff level. English SPRINGER INT PUBL AG Quantitative analysis of galactose using LDI-TOF MS based on a TiO2 nanowire chip Article 10.1186/s40543-021-00300-7 1 JOURNAL OF ANALYTICAL SCIENCE AND TECHNOLOGY, v.12, no.1 JOURNAL OF ANALYTICAL SCIENCE AND TECHNOLOGY 12 1 Y scie scopus 000714019900001 2-s2.0-85118560694 Chemistry, Analytical Chemistry Article IONIZATION MASS-SPECTROMETRY LASER-DESORPTION IONIZATION GLUCOSE MILK PEROXIDASE PRODUCTS LACTOSE 3-diaminophenazine Galactosemia Matrix-assisted laser desorption ionization time-of-flight mass spectrometry TiO2 nanowire chip Galactose Reduction power o-phenylene diamine 2