Park, T.-J. Hong, H. Kim, M.-S. Park, J.-S. Chi, W.-J. Kim, S.-Y. 2024-01-19T13:31:46Z 2024-01-19T13:31:46Z 2022-01-10 2021-11 1420-3049 https://pubs.kist.re.kr/handle/201004/116197 Biorenovation, a microbial enzyme-assisted degradation process of precursor compounds, is an effective approach to unraveling the potential bioactive properties of the derived compounds. In this study, we obtained a new compound, prunetin 4′-O-phosphate (P4P), through the biorenovation of prunetin (PRN), and investigated its anti-inflammatory effects in lipopolysaccharide (LPS)-treated RAW 264.7 macrophage cells. The anti-inflammatory effect of P4P was evaluated by measuring the production of prostaglandin-E2 (PGE2), nitric oxide (NO), which is an inflammation-inducing factor, and related cytokines such as tumor necrosis factor-α (TNFα), interleukin-1β (IL1β), and interleukin-6 (IL6). The findings demonstrated that P4P was non-toxic to cells, and its inhibition of the secretion of NO―as well as pro-inflammatory cytokines―was concentration-dependent. A simultaneous reduction in the protein expression level of pro-inflammatory proteins such as cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) was observed. Moreover, the phosphorylation of mitogen-activated protein kinases (MAPKs) such as extracellular signal-regulated kinases (ERKs), c-Jun N-terminal kinase (JNK), p38 MAPK (p38), and nuclear factor kappa B (NFκB) was downregulated. To conclude, we report that biorenovation-based phosphorylation of PRN improved its anti-inflammatory activity. Cell-based in vitro assays further confirmed that P4P could be applied in the development of anti-inflammatory therapeutics. ？ 2021 by the authors. Licensee MDPI, Basel, Switzerland. English MDPI Prunetin 4′-o-phosphate, a novel compound, in raw 264.7 macrophages exerts anti-inflammatory activity via suppression of map kinases and the nfκb pathway Article 10.3390/molecules26226841 1 Molecules, v.26, no.22 Molecules 26 22 scie scopus 000725393900001 2-s2.0-85119624301 Biochemistry & Molecular Biology Chemistry, Multidisciplinary Biochemistry & Molecular Biology Chemistry Article SIGNALING PATHWAY MOLECULAR-MECHANISMS GENE-EXPRESSION IN-VITRO INHIBITION INFLAMMATION ACTIVATION GENISTEIN INNATE AGENTS Anti-inflammatory activities Biorenovation MAPK signaling Phosphorylation Prunetin Prunetin 4′-O-phosphate