Kim, Seongchan Kim, Shin Young Rho, Seung Joon Kim, Seung Hoon Song, So Hyang Kim, Chi Hong Lee, Hyojin Kim, Sung Kyoung 2024-01-19T13:31:50Z 2024-01-19T13:31:50Z 2022-01-10 2021-11 2045-2322 https://pubs.kist.re.kr/handle/201004/116201 Oxidative stress plays important roles in inflammatory responses during acute lung injury (ALI). Recently, nanoconstruct (Nano)-based drug-delivery systems have shown promise in many models of inflammation. In this study, we evaluated the anti-inflammatory effects of N-acetylcysteine (NAC) loaded in a biocompatible Nano using a rat model of ALI. We synthesized a Nano with a good NAC-releasing capacity using porous silica Nano, which was used to produce Nano/NAC complexes. For in vivo experiments, Sprague-Dawley rats were intraperitoneally administered NAC or Nano/NAC 30 min after intratracheal instillation of lipopolysaccharide. After 6 h, bronchoalveolar lavage fluids and lung tissues were collected. The anti-oxidative effect of the Nano/NAC complex was confirmed by demonstrating reduced levels of reactive oxygen species after treatment with the Nano/NAC in vitro. In vivo experiments also showed that the Nano/NAC treatment may protect against LPS-induced ALI thorough anti-oxidative and anti-inflammatory effects, which may be attributed to the inactivation of the NF-kappa B and MAPK pathways. In addition, the effects of Nano/NAC treatment were shown to be superior to those of NAC alone. We suggest the therapeutic potential of Nano/NAC treatment as an anti-inflammatory agent against ALI. Furthermore, our study can provide basic data for developing nanotechnology-based pharmacotherapeutics for ALI. English Nature Publishing Group Biocompatible N-acetyl-nanoconstruct alleviates lipopolysaccharide-induced acute lung injury in vivo Article 10.1038/s41598-021-01624-5 1 Scientific Reports, v.11, no.1 Scientific Reports 11 1 Y scie scopus 000722270000014 2-s2.0-85119689527 Multidisciplinary Sciences Science & Technology - Other Topics Article ACUTE RESPIRATORY-DISTRESS MESOPOROUS SILICA NANOPARTICLES OXIDATIVE STRESS DOUBLE-BLIND ACETYLCYSTEINE DRUG BIODISTRIBUTION PHARMACOKINETICS PHARMACOTHERAPY CLEARANCE