Jung, J. Bong, J.-H. Sung, J.S. Lee, S.J. Lee, M. Kang, M.-J. Jose, J. Pyun, J.-C. 2024-01-19T13:33:31Z 2024-01-19T13:33:31Z 2022-01-10 2021-10 1043-1802 https://pubs.kist.re.kr/handle/201004/116308 In this study, the binding domains for fluorescent dyes were presented that could be used as synthetic peptides or fusion proteins. Fv-antibodies against two fluorescent dyes (fluorescein and rhodamine B) were screened from the Fv-antibody library, which was prepared on the outer membrane of Escherichia coli using the autodisplay technology. Two clones with binding activities to each fluorescent dye were screened separately from the library using flow cytometry. The binding activity of the screened Fv-antibodies on the outer membrane was analyzed using fluorescent imaging with the corresponding fluorescent dyes. The CDR3 regions of the screened Fv-antibodies (11 amino acid residues) were synthesized into peptides, and each peptide was analyzed for its binding activity to each fluorescent dye using fluorescence resonance energy transfer (FRET) experiments. These CDR3 regions were demonstrated to have a binding activity to each fluorescent dye when the regions were co-expressed as a fusion protein with Z-domain. ？ 2021 American Chemical Society. English American Chemical Society Fluorescein and Rhodamine B-Binding Domains from Autodisplayed Fv-Antibody Library Article 10.1021/acs.bioconjchem.1c00376 1 Bioconjugate Chemistry, v.32, no.10, pp.2213 - 2223 Bioconjugate Chemistry 32 10 2213 2223 N scie scopus 000710929100011 2-s2.0-85117504554 Biochemical Research Methods Biochemistry & Molecular Biology Chemistry, Multidisciplinary Chemistry, Organic Biochemistry & Molecular Biology Chemistry Article HILL EQUATION DIAGNOSIS PROTEINS OUTER-MEMBRANE Fv antibodies fluoresein and Rhodamine B-binding FRET CDR3