에르덴돌고르 에르덴-오치르 Shin, Bok-Kyu Huda, Md Nazmul Lee, Eun Ha Song, Dae-Geun Jung, Choonkyun Pan, Cheol-Ho 2024-01-19T13:33:45Z 2024-01-19T13:33:45Z 2022-01-10 2021-10 2045-8827 https://pubs.kist.re.kr/handle/201004/116323 Although diatoms have been utilized as a cellular factory to produce biopharmaceuticals, recombinant proteins, and biofuels, only a few numbers of gene promoters are available. Therefore, the development of novel endogenous promoters is essential for the production of a range of bioactive substances. Here, we characterized the activities of endogenous promoters glyceraldehyde-3-phosphate dehydrogenase (GapC1) and glutamine synthetase (GS) of Phaeodactylum tricornutum using green fluorescent protein (GFP) under different culture conditions. Compared with the widely used fucoxanthin chlorophyll-binding protein A (fcpA) promoter, the GS promoter constitutively drove the expression of GFP throughout all growth phases of P. tricornutum, regardless of culture conditions. Additionally, the GFP level driven by the GapC1 promoter was the highest at the log phase, similar to the fcpA promoter, and increased light and nitrogen-starvation conditions reduced GFP levels by inhibiting promoter activity. These results suggested that the GS promoter could be utilized as a strong endogenous promoter for the genetic engineering of P. tricornutum. English WILEY Characterization of endogenous promoters of GapC1 and GS for recombinant protein expression in Phaeodactylum tricornutum Article 10.1002/mbo3.1239 1 MICROBIOLOGYOPEN, v.10, no.5 MICROBIOLOGYOPEN 10 5 N scie scopus 000712037200014 2-s2.0-85117949014 Microbiology Microbiology Article BETA-CARBONIC ANHYDRASE TRANSCRIPTION FACTOR DIATOM TRANSFORMATION SEQUENCES ELEMENTS VARIETY REVEALS MOTIFS TOOLS diatom endogenous promoter glutamine synthetase glyceraldehyde-3-phosphate dehydrogenase Phaeodactylum tricornutum