Lee, Jii Bum Kim, Kyung-A Cho, Ho Yeon Kim, DooA Kim, Won Kyu Yong, Dongeun Lee, Hyukmin Yoon, Sang Sun Han, Dai Hoon Han, Yoon Dae Paik, Soonmyung Jang, Mi Kim, Han Sang Ahn, Joong Bae 2024-01-19T13:33:55Z 2024-01-19T13:33:55Z 2022-01-10 2021-10 2045-2322 https://pubs.kist.re.kr/handle/201004/116332 Recent evidence suggests that Fusobacterium nucleatum (Fn) is associated with the development and progression of colorectal cancer. We aimed to delineate the clinical implications of Fn in metastatic colon cancer. We performed quantitative polymerase chain reaction (qPCR) using DNA samples from synchronous metastatic colon cancer patients with either formalin-fixed paraffin-embedded (FFPE) archival primary site tumor samples or fresh colon tissues. Progression-free survival (PFS)1 and PFS2 were defined as PFS of first- and second-line palliative settings. qPCR for Fn was successfully performed using 112 samples (FFPE, n = 61; fresh tissue, n = 51). Forty-one and 68 patients had right-sided and left-sided colon cancer, respectively. Patients with Fn enriched right-sided colon cancers had shorter PFS1 (9.7 vs. 11.2 months) than the other subgroups (HR 3.54, 95% confidence interval [CI] 1.05-11.99; P = 0.04). Fn positive right-sided colon was also associated with shorter PFS2 (3.7 vs. 6.7 months; HR 2.34, 95% CI 0.69-7.91; P = 0.04). In the univariate analysis, PFS1 was affected by differentiation and Fn positive right-sided colon cancer. The multivariate analysis showed that differentiation (HR 2.68, 95% CI 1.40-5.14, P = 0.01) and Fn positive right-sided colon (HR 0.40, 95% CI 0.18-0.88, P = 0.02) were associated with PFS1. Fn enrichment in right sided colon was not associated with overall survival (OS). Fn enrichment has significantly worse prognosis in terms of PFS1 and PFS2 in patients with right-sided metastatic colon cancers. English Nature Publishing Group Association between Fusobacterium nucleatum and patient prognosis in metastatic colon cancer Article 10.1038/s41598-021-98941-6 1 Scientific Reports, v.11, no.1 Scientific Reports 11 1 Y scie scopus 000706830500095 2-s2.0-85117204758 Multidisciplinary Sciences Science & Technology - Other Topics Article ADVANCED COLORECTAL-CANCER MOLECULAR-FEATURES SURVIVAL