Lee, Seon Yong Choi, Sang-Hun Lee, Min Seok Kurmashev, Amanzhol Lee, Hae Nim Ko, Young-Gyu Lee, Kanghun Jeong, Sohee Seong, Jihye Kang, Joo H. Kim, Hyunggee 2024-01-19T13:34:04Z 2024-01-19T13:34:04Z 2022-01-10 2021-10 0892-6638 https://pubs.kist.re.kr/handle/201004/116343 Glioblastoma (GBM) is a refractory disease that has a highly infiltrative characteristic. Over the past decade, GBM perivascular niche (PVN) has been described as a route of dissemination. Here. we investigated that trailed membrane structures, namely retraction fibers (RFs). are formed by perivascular extracellular matrix (ECM) proteins. By using the anatomical GBM database, we validated that the ECM-related genes were highly expressed in the cells within the PVN where fibronectin (FN) induced RF formation. By disrupting candidates of FN-binding integrins, integrin alpha 5 beta 1 was identified as the main regulator of RF formation. De novo RFs were produced at the trailing edge, and focal adhesions were actively localized in RFs, indicating that adhesive force makes RFs remain at the bottom surface. Furthermore, we observed that GBM cells more frequently migrated along the residual RFs formed by preceding cells in microfluidic channels in comparison to those in the channels without RFs, suggesting that the infiltrative characteristics GBM could be attributed to RFs formed by the preceding cells in concert with chemoattractant cues. Altogether, we demonstrated that shedding membrane structures of GBM cells are maintained by FN-integrin alpha 5 beta 1 interaction and promoted their motility. English WILEY MIGRASOME COMMUNICATION Retraction fibers produced by fibronectin-integrin alpha 5 beta 1 interaction promote motility of brain tumor cells Article 10.1096/fj.202100452RR 1 FASEB JOURNAL, v.35, no.10 FASEB JOURNAL 35 10 scie scopus 000702238200044 2-s2.0-85115828902 Biochemistry & Molecular Biology Biology Cell Biology Biochemistry & Molecular Biology Life Sciences & Biomedicine - Other Topics Cell Biology Article MIGRASOME COMMUNICATION cell migration lihronectin integrin alpha 5 beta 1 perivascular niche retraction fiber