Park, In Su Kim, Sang-Heon Han, Jeong Woo Ko, Young Gun Chung, Eunna Kim, Soo Hyun 2024-01-19T13:38:33Z 2024-01-19T13:38:33Z 2022-03-07 2007 1013-9826 https://pubs.kist.re.kr/handle/201004/116405 Porosity and pore size are needed for successful cell seeding and proliferation into porous scaffolds. This study was focused on a hydrogel-seeding method to improve cell adhesion and proliferation in tubular porous scaffolds for vascular grafts application. Tubular scaffolds were fabricated from a biodegradable elastic polymer, poly(L-lactide-co-epsilon-caprolactone) (PLCL) (50:50, Mn 1.58x10(5)). by an extrusion-particulate leaching method. Vascular smooth muscle cells (VSMCs) were dispersed in collagen hydrogel and then seeded into the tubular PLCL scaffolds having various pore sizes, 50-100 mu m, 100-200 mu m, and 300-500 mu m, respectively. As a result, the efficiency of cell adhesion and proliferation was dependent on the porc size of the scaffolds. Especially, the cell proliferation efficiency was improved by using the hydrogel-seeding method as compared with by using a previously established method. In summary, this study demonstrates that the efficiency of cell adhesion and proliferation was dependent on the pore size of the scaffolds in the hydrogel-seeding method. English TRANS TECH PUBLICATIONS LTD Optimization of scaffold for a successful hydrogel-seeding method for vascular tissue engineering Conference 10.4028/www.scientific.net/KEM.342-343.333 1 7th Asian Symposium on Biomedical Materials (ASBM7), v.342-343, pp.333 - + 7th Asian Symposium on Biomedical Materials (ASBM7) 342-343 333 + SZ Cheju Isl, SOUTH KOREA 2006-08-20 ASBM7: ADVANCED BIOMATERIALS VII 000246657900084 2-s2.0-34147158990 Proceedings Paper