Lee, So Eun Jung, Youngmee Kim, Soo Hyun Kim, Sang-Heon Rhie, Jong Won Kim, Young Ha Min, Byoung Goc 2024-01-19T13:38:36Z 2024-01-19T13:38:36Z 2022-03-07 2007 1013-9826 https://pubs.kist.re.kr/handle/201004/116408 In cartilage tissue engineering, as a cell source, adult stem cells are very attractive for clinical applications. Recent studies suggest that human adipose tissue-derived stromal cells (ASCs) have multilineage potential similar to bone marrow-derived stromal cells (BMSCs). ASCs are obtained from adipose tissue easily isolated by suction-assisted lipectomy in various body parts. Also, as one of major factors of cartilage tissue engineering, scaffolds have an important role in cartilage formation. Poly(L-lactide-co-epsilon-carprolactone) scaffolds have physiological activity, biodegradability. high cell affinity, and mechano-activity. The object of this study is cartilaginous tissue formation using highly elastic PLCL scaffolds and ASCs in vitro and in vivo. Poly,(L-lactide-co-epsilon:-carprolactone) copolymers were synthesized from lactide and epsilon-carprolactone in the presence of stannous octoate as catalyst. The scaffolds with 85% porosity and 300-500 mu m pore size were fabricated by gel-pressing method. ASCs were seeded on scaffolds and cultured for 21 days in vitro. Cell/polymer constructs were characterized by reverse transcriptase-polymerase chain reaction for confirming differentiation to chondrocytes onto PLCL scaffolds. Also, for examining cartilaginous tissue formation in vivo, ASCs seeded scaffolds which were induced chondrogenesis for 2 weeks were implanted in nude mice subcutaneously for up to 8 weeks. Histological studies showed that implants partially developed cartilaginous tissue within lacunae. And there was an accumulation of sulfated glycoaminoglycans. Immunohistochemical analysis revealed that implants were positively stained for specific extracellular matrix. These results indicate that ASCs and PLCL scaffols Could be used to cartilage tissue engineering. English TRANS TECH PUBLICATIONS LTD Cartilaginous tissue formation with an elastic PLCL scaffold and human adipose tissue-derived stromal cells Conference 10.4028/www.scientific.net/KEM.342-343.385 1 7th Asian Symposium on Biomedical Materials (ASBM7), v.342-343, pp.385 - + 7th Asian Symposium on Biomedical Materials (ASBM7) 342-343 385 + SZ Cheju Isl, SOUTH KOREA 2006-08-20 ASBM7: ADVANCED BIOMATERIALS VII 000246657900097 2-s2.0-34147162400 Proceedings Paper