Lee, Soo-Hong Chung, Hyung Min Kim, Young Ha Kim, Soo-Hyun 2024-01-19T13:38:43Z 2024-01-19T13:38:43Z 2022-03-07 2007 1013-9826 https://pubs.kist.re.kr/handle/201004/116416 We have developed a direct conjugation method of RGD (Arg-Gly-Asp) into polylactide (PLA) using co-solvent system, which showed high reaction yield compared to one solvent system presumably due to good solubility of reactants. First, hydroxyl groups a the end of PLA were changed into carboxyl acid by ring opening reaction of succinic anhydride. For a coupling reaction with high solubility, RGD was dissolved in water and then PLA in dioxane Was added into RGD solution in the presence of 1-(3-dimenthylaminopropyl)3-ethylcarbodiimide hydrochloride (EDC). The conjugation of RGD was confirmed by H-1-NMR, GPC and elementary analysis. The RGD conjugated PLA (RGD-PLA) on poly(lactide-co-glycolide) (PLGA) film significantly increased the number of attached cells compared to PLGA without RGD-PLA, while it did not change static contact angle of PLGA. This result demonstrated this conjugation method would be a useful tool to increase cell affinity of biomaterials without change in hydrophilic/hydrophobic property for tissue engineering applications. English TRANS TECH PUBLICATIONS LTD RGD direct conjugation using co-solvent system to increase cell attachment on poly(lactide-co-glycolide) without change in hydrophilic/hydrophobic property Conference 10.4028/www.scientific.net/KEM.342-343.157 1 7th Asian Symposium on Biomedical Materials (ASBM7), v.342-343, pp.157 - + 7th Asian Symposium on Biomedical Materials (ASBM7) 342-343 157 + SZ Cheju Isl, SOUTH KOREA 2006-08-20 ASBM7: ADVANCED BIOMATERIALS VII 000246657900040 2-s2.0-34147098116 Proceedings Paper