Wi, Ji Hun Heo, Cheol Ho Gwak, HyeRan Jung, Cheulhee Kim, So Yeon 2024-01-19T14:00:20Z 2024-01-19T14:00:20Z 2021-10-21 2021-09-16 1520-6106 https://pubs.kist.re.kr/handle/201004/116461 Cellular senescence is the irreversible cell cycle arrest in response to various types of stress. Although the plasma membrane and its composition are significantly affected by cellular senescence, detailed studies on the physical properties of the plasma membrane have shown inconclusive results. In this study, we utilized both ensemble and single-molecule fluorescence imaging to investigate how membrane properties, such as fluidity, hydrophobicity, and ganglioside GM1 level are affected by cellular senescence. The diffusion coefficient of lipid probes, as well as the type of diffusion determined by an exponent alpha, which is the slope of the log-log plot of mean squared displacement as a function of time lag, were analyzed. We found that the number of molecules with a lower diffusion coefficient increased as cells became senescent. The changes in the population with a lower diffusion coefficient, observed after methyl-beta-cyclodextiin treatment, and the increase in ceramide levels, detected using a ceramide-specific antibody, suggest that ceramide-rich lipid rafts were enhanced in senescent cells. Our results emphasize the importance of membrane properties in cellular senescence and might serve as a base for in-depth studies to determine how such domains facilitate the signaling pathway specific to cellular senescence. English AMER CHEMICAL SOC AGE-RELATED-CHANGES PLASMA-MEMBRANE CHOLESTEROL DEPLETION LIPID RAFTS MODEL DIFFUSION CERAMIDE CYCLODEXTRINS ORGANIZATION MICRODOMAINS Probing Physical Properties of the Cellular Membrane in Senescent Cells by Fluorescence Imaging Article 10.1021/acs.jpcb.1c05403 1 JOURNAL OF PHYSICAL CHEMISTRY B, v.125, no.36, pp.10182 - 10194 JOURNAL OF PHYSICAL CHEMISTRY B 125 36 10182 10194 scie scopus 000697332700012 2-s2.0-85114877015 Chemistry, Physical Chemistry Article AGE-RELATED-CHANGES PLASMA-MEMBRANE CHOLESTEROL DEPLETION LIPID RAFTS MODEL DIFFUSION CERAMIDE CYCLODEXTRINS ORGANIZATION MICRODOMAINS