Park, Jin-Soo Kim, Da-Eun Hong, Sung-Chul Kim, Seung-Young Kwon, Hak Cheol Hyun, Chang-Gu Choi, Jaeyoung 2024-01-19T14:01:35Z 2024-01-19T14:01:35Z 2021-10-21 2021-09 2076-2607 https://pubs.kist.re.kr/handle/201004/116541 Streptomyces spp. have been major contributors of novel natural products that are used in many application areas. We found that the nojirimycin (NJ) producer JCM 3382 has antimicrobial activity against Staphylococcus aureus via cellular degradation. Genome analysis revealed 30 biosynthetic gene clusters, including those responsible for producing antibiotics, including an azasugar NJ. In-depth MS/MS analysis confirmed the production of 1-deoxynojirimycin (DNJ) along with NJ. In addition, the production of tambromycins, setomimycin, and linearmycins was verified by spectroscopic analyses, including LC-MS and NMR. The distribution of the clusters of genes coding for antibiotics in 2061 Streptomyces genomes suggested potential producers of tambromycin, setomimycin, and linearmycin. For a DNJ gene cluster, homologs of gabT1 and gutB1 were commonly found; however, yktC1 was identified in only 112 genomes. The presence of several types of clusters suggests that different strains may produce different types of azasugars. Chemical-profile-inspired comparative genome analysis may facilitate a more accurate assessment of the biosynthetic potential to produce secondary metabolites. English MDPI Genome Analysis of Streptomyces nojiriensis JCM 3382 and Distribution of Gene Clusters for Three Antibiotics and an Azasugar across the Genus Streptomyces Article 10.3390/microorganisms9091802 1 MICROORGANISMS, v.9, no.9 MICROORGANISMS 9 9 scie scopus 000700015200001 2-s2.0-85120902713 Microbiology Microbiology Article NATURAL-PRODUCT DISCOVERY BIOSYNTHESIS IDENTIFICATION NOJIRIMYCIN 1-DEOXYNOJIRIMYCIN ANNOTATION ACTIVATION PEPTIDE Streptomyces nojiriensis JCM 3382 antibiotics nojirimycin biosynthetic gene cluster secondary metabolism comparative genomics