Suhaeri, Muhammad Kim, Young-Min Yunarti, Rika Tri Song, Soo-Chang 2024-01-19T14:03:08Z 2024-01-19T14:03:08Z 2021-10-21 2021-08 1773-2247 https://pubs.kist.re.kr/handle/201004/116640 Camptothecin (CPT) has been used as antitumor drug against a wide range of cancer cells. However, its clinical application is greatly hindered by insolubility and instability issues under physiological condition. Therefore, an appropriate CPT administration technique directed for living system is greatly anticipated. In this study, a delivery method for CPT in the form of thermoresponsive system was prescribed. A conjugate of cyclotriphosphazene and CPT was synthesized by substituting hexachlorocyclotriphosphazene with sodium salt of methoxy-poly (ethylene glycol) (Mw = 350), 20-O-trifluoroglycinylCPT, and isoleucine ethyl ester, respectively. The resulting cyclotriphosphazene-CPT was characterized via multinuclear (1H and 31P) NMR as well as FT-IR. The current conjugate showed temperature induced phase transition (solution to suspension) with a lower critical solution temperature at 31 degrees C. Our result indicated that the stability issue related to the use of CPT in aqueous solution could be handled by acylation at 20-OH moiety. Additionally, antitumor activity of cyclotriphosphazene-CPT, to some extent, was found to be more profound than that of CPT alone as evaluated against human colorectal cancer cell HCT-116. Altogether, the current cyclotriphosphazene-CPT conjugate might offer a facile method in delivering CPT as a minimally invasive system for treating cancer. English ELSEVIER TOPOISOMERASE-I MECHANISM Thermoresponsive and suspension forming cyclotriphosphazene conjugate for delivery vehicle of antitumor drug camptothecin Article 10.1016/j.jddst.2020.102049 1 JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY, v.64 JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY 64 scie scopus 000674606800007 2-s2.0-85116958285 Pharmacology & Pharmacy Pharmacology & Pharmacy Article TOPOISOMERASE-I MECHANISM Drug delivery Cyclotriphosphazene Camptothecin Conjugate Thermoresponsive Antitumor