Jang, Seonghoe Cho, Jwa-Yeong Do, Gyung-Ran Kang, Yeeun Li, Hsing-Yi Song, Jaeeun Kim, Ho-Youn Kim, Beom-Gi Hsing, Yue-Ie 2024-01-19T14:03:20Z 2024-01-19T14:03:20Z 2021-10-21 2021-08 1661-6596 https://pubs.kist.re.kr/handle/201004/116654 Leaf angle and grain size are important agronomic traits affecting rice productivity directly and/or indirectly through modulating crop architecture. OsBC1, as a typical bHLH transcription factor, is one of the components comprising a complex formed with LO9-177 and OsBUL1 contributing to modulation of rice leaf inclination and grain size. In the current study, two homologues of OsBC1, OsBCL1 and OsBCL2 were functionally characterized by expressing them under the control of OsBUL1 promoter, which is preferentially expressed in the lamina joint and the spikelet of rice. Increased leaf angle and grain length with elongated cells in the lamina joint and the grain hull were observed in transgenic rice containing much greater gibberellin A(3) (GA(3)) levels than WT, demonstrating that both OsBCL1 and OsBCL2 are positive regulators of cell elongation at least partially through increased GA biosynthesis. Moreover, the cell elongation was likely due to cell expansion rather than cell division based on the related gene expression and, the cell elongation-promoting activities of OsBCL1 and OsBCL2 were functional in a dicot species, Arabidopsis. English MDPI Modulation of Rice Leaf Angle and Grain Size by Expressing OsBCL1 and OsBCL2 under the Control of OsBUL1 Promoter Article 10.3390/ijms22157792 1 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v.22, no.15 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 22 15 N scie scopus 000681909100001 2-s2.0-85110647676 Biochemistry & Molecular Biology Chemistry, Multidisciplinary Biochemistry & Molecular Biology Chemistry Article CELL ELONGATION LAMINA JOINT PROTEIN GIBBERELLIN GENE BRASSINOSTEROIDS REGULATOR ARCHITECTURE RESPONSES HOMOLOG bHLH cell elongation leaf inclination lamina joint transcription factor rice