Kwon, Jaeyoung Kim, Min Jee Kim, D.-C. Kwon, H. Ryu, S.M. Shim, S.H. Guo, Y. Hong, S.-B. Yim, J.H. Kim, Y.-C. Oh, H. Lee, D. 2024-01-19T14:03:29Z 2024-01-19T14:03:29Z 2021-09-02 2021-08 0045-2068 https://pubs.kist.re.kr/handle/201004/116664 Inflammation is a vital process that maintains tissue homeostasis. However, it is widely known that uncontrolled inflammation can contribute to the development of various diseases. This study aimed to discover anti-inflammatory metabolites from Penicillium bialowiezense. Seven spiroditerpenoids, including two new compounds, breviones P and Q (1 and 2), were isolated and characterized by various spectroscopic and spectrometric methods. All isolated compounds were initially tested for their inhibitory effects against lipopolysaccharide-induced nitric oxide (NO) production in RAW 264.7 macrophages. Of these, brevione A (3) exhibited this activity with a half-maximal inhibitory concentration value of 9.5 μM. Further mechanistic studies demonstrated that 3 could suppress the expression of pro-inflammatory cytokines and mediators, such as NO, prostaglandin E2, interleukin (IL)-1β, tumor necrosis factor-α, IL-6, and IL-12 by inhibiting the activation of nuclear factor-kappa B and c-Jun N-terminal kinase. ？ 2021 Elsevier Inc. English Academic Press Inc. Anti-inflammatory spiroditerpenoids from Penicillium bialowiezense Article 10.1016/j.bioorg.2021.105012 1 Bioorganic Chemistry, v.113 Bioorganic Chemistry 113 N scie scopus 000663790900006 2-s2.0-85107051480 Biochemistry & Molecular Biology Chemistry, Organic Biochemistry & Molecular Biology Chemistry Article NF-KAPPA-B LPS-INDUCED INFLAMMATION BREVIANE SPIRODITERPENOIDS MECHANISMS PATHWAYS Anti-inflammatory effect Brevione Penicillium bialowiezense Spiroditerpenoid