Jun, Indong Han, Hyung-Seop Lee, Jae Won Lee, Kyungwoo Kim, Yu-Chan Ok, Myoung-Ryul Seok, Hyun-Kwang Kim, Young Jun Song, In-Seok Shin, Heungsoo Edwards, James R. Lee, Kuen Yong Jeon, Hojeong 2024-01-19T14:03:47Z 2024-01-19T14:03:47Z 2021-10-21 2021-07 2050-750X https://pubs.kist.re.kr/handle/201004/116683 The utilization of cell-manipulating techniques reveals information about biological behaviors suited to address a wide range of questions in the field of life sciences. Here, we introduced an on/off switchable physical stimuli technique that offers precise stimuli for reversible cell patterning to allow regulation of the future direction of adherent cellular behavior by leveraging enzymatically degradable alginate hydrogels with defined chemistry and topography. As a proof of concept, targeted muscle cells adherent to TCP exhibited a reshaped structure when the hydrogel-based physical stimuli were applied. This simple tool offers easy manipulation of adherent cells to reshape their morphology and to influence future direction depending on the characteristics of the hydrogel without limitations of time and space. The findings from this study are broadly applicable to investigations into the relationships between cells and physiological extracellular matrix environments as well as has potential to open new horizons for regenerative medicine with manipulated cells. English Royal Society of Chemistry On/off switchable physical stimuli regulate the future direction of adherent cellular fate Article 10.1039/d1tb00908g 1 Journal of Materials Chemistry B, v.9, no.27, pp.5560 - 5571 Journal of Materials Chemistry B 9 27 5560 5571 N scie scopus 000665801900001 2-s2.0-85110499724 Materials Science, Biomaterials Materials Science Article SKELETAL-MUSCLE TISSUE MATRIX STIFFNESS IN-VIVO HYDROGELS DIFFERENTIATION BIOMATERIALS ACTIVATION EXPRESSION STRATEGIES ADHESION physical stimuli cell-manipulating techniques direct contact pressing alginate hydrogel micro patterning