Kang, GY Han, GY Kang, JY Cho, IH Park, HH Paek, SH Kim, TS 2024-01-19T14:09:17Z 2024-01-19T14:09:17Z 2022-03-07 2005 https://pubs.kist.re.kr/handle/201004/116764 This paper reports a label-free detection of bin using microfabricated self-actuating PZT myoglobin cantilever. Electrical detection of antibody-antigen interaction on the surface was achieved through measuring the resonant frequency of cantilever. On Au surface of cantilever, biotinylated antibody bound to the streptavidin layer. We immobilized site-directed antibody through reducing the binding region to hinge region, which resolves the random arrangement of the biotinylated antibody and increases the number of binding complexes. This protocol enhanced the performance of analytical sensitivity about ten times, and the experimental results demonstrated this biosensor can be used for diagnosis of acute myocardial infarction in clinical range of tens of ng/ml. English IEEE Label-free protein assay with site-directly immobilized antibody using self-actuating PZT cantilever Conference 1 13th International Conference on Solid-State Sensors, Actuators and Microsystems, pp.1812 - 1815 13th International Conference on Solid-State Sensors, Actuators and Microsystems 1812 1815 US Seoul, SOUTH KOREA 2005-06-05 Transducers '05, Digest of Technical Papers, Vols 1 and 2 000232189100448 2-s2.0-27544452647 Proceedings Paper