Odermatt, Pascal D. Miettinen, Teemu P. Lemiere, Joel Kang, Joon Ho Bostan, Emrah Manalis, Scott R. Huang, Kerwyn Casey Chang, Fred 2024-01-19T14:31:34Z 2024-01-19T14:31:34Z 2021-10-21 2021-06 2050-084X https://pubs.kist.re.kr/handle/201004/116865 Intracellular density impacts the physical nature of the cytoplasm and can globally affect cellular processes, yet density regulation remains poorly understood. Here, using a new quantitative phase imaging method, we determined that dry-mass density in fission yeast is maintained in a narrow distribution and exhibits homeostatic behavior. However, density varied during the cell cycle, decreasing during G2, increasing in mitosis and cytokinesis, and dropping rapidly at cell birth. These density variations were explained by a constant rate of biomass synthesis, coupled to slowdown of volume growth during cell division and rapid expansion post-cytokinesis. Arrest at specific cell-cycle stages exacerbated density changes. Spatially heterogeneous patterns of density suggested links between density regulation, tip growth, and intracellular osmotic pressure. Our results demonstrate that systematic density variations during the cell cycle are predominantly due to modulation of volume expansion, and reveal functional consequences of density gradients and cell-cycle arrests. English ELIFE SCIENCES PUBLICATIONS LTD Variations of intracellular density during the cell cycle arise from tip-growth regulation in fission yeast Article 10.7554/eLife.64901 1 ELIFE, v.10 ELIFE 10 N scie scopus 000667500900001 2-s2.0-85108895712 Biology Life Sciences & Biomedicine - Other Topics Article MASS MEASUREMENTS SHOW DIVISION CYCLE SCHIZOSACCHAROMYCES-POMBE SINGLE CELLS VOLUME SEPTATION OSCILLATIONS SWELL