Ryu, Yeon Hee Moon, Suk-Ho. Kim, Ki Joo Jun, Young Joon Oh, Deuk Young Kim, Sang-Heon Rhie, Jong-Won 2024-01-19T14:32:45Z 2024-01-19T14:32:45Z 2021-09-05 2021-06 1738-2696 https://pubs.kist.re.kr/handle/201004/116935 Background: Angiogenesis and vasculogenesis are essential processes for successful tissue regeneration in tissue engineering and regenerative medicine. The adipose-derived stromal vascular fraction (SVF) is not only a source of adipose stem cells (ASC) but also a suitable source of microvascular endothelial cells because it is a rich capillary network. So, we propose a new hypothesis for isolating adipose-derived human microvascular endothelial cells (HMVEC-A) from the SVF and developed a dual isolation system that isolates two cell types from one tissue. Method: To isolate HMVEC-A, we analyzed the supernatant discarded when ASC is isolated from the adipose-derived SVF. Based on this analysis, we assumed that the SVF adherent to the bottom of the culture plate was divided into two fractions: the stromal fraction as the ASC-rich fraction, and the vascular fraction (VF) as the endothelial cells-rich fraction floating in the culture supernatant. VF isolation was optimized and the efficiency was compared, and the endothelial cells characteristics of HMVEC-A were confirmed by flow cytometric analysis, immunocytochemistry (ICC), a DiI-acetylated low-density lipoprotein (DiI-Ac-LDL) uptake, and in vitro tube formation assay. Results: Consistent with the hypothesis, we found a large population of HMVEC-A in the VF and isolated these HMVEC-A by our isolation method. Additionally, this method had higher yields and shorter doubling times than other endothelial cells isolation methods and showed typical morphological and phenotypic characteristics of endothelial cells. Conclusion: Cells obtained by the method according to our hypothesis can be applied as a useful source for studies such as tissue-to-tissue networks, angiogenesis and tissue regeneration, patient-specific cell therapy, and organoid chips. English KOREAN TISSUE ENGINEERING REGENERATIVE MEDICINE SOC A Novel Hypothesis and Characterization to Isolate Microvascular Endothelial Cells Simultaneously with Adipose-Derived Stem Cells from the Human Adipose-Derived Stromal Vascular Fraction Article 10.1007/s13770-021-00332-5 1 TISSUE ENGINEERING AND REGENERATIVE MEDICINE, v.18, no.3, pp.429 - 440 TISSUE ENGINEERING AND REGENERATIVE MEDICINE 18 3 429 440 scie scopus kci ART002722101 000641670100002 2-s2.0-85107258715 Cell & Tissue Engineering Engineering, Biomedical Cell Biology Engineering Article EXTRACELLULAR-MATRIX PROGENITOR CELLS TISSUE DIFFERENTIATION ANGIOGENESIS NETWORKS OBESITY Microvascular endothelial cells Stromal vascular fraction Adipose tissue Cell isolation