Park, Hee Ho Kim, Hyelim Lee, Han Sol Seo, Eun U. Kim, Ji-Eun Lee, Jee-Hyun Mun, Yong-Hyeon Yoo, So-Yeol An, Jiseon Yun, Mi-Young Kang, Nae-Won Kim, Dae-Duk Na, Dong Hee Hong, Kyung Soo Jang, Jong Geol Ahn, June Hong Bae, Jong-Sup Song, Gyu Yong Lee, Jae-Young Kim, Hong Nam Lee, Wonhwa 2024-01-19T14:32:56Z 2024-01-19T14:32:56Z 2021-09-04 2021-06 0142-9612 https://pubs.kist.re.kr/handle/201004/116945 The rapid spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) on a global scale urges prompt and effective countermeasures. Recently, a study has reported that coronavirus disease-19 (COVID-19), the disease caused by SARS-CoV-2 infection, is associated with a decrease in albumin level, an increase in NETosis, blood coagulation, and cytokine level. Here, we present drug-loaded albumin nanoparticles as a therapeutic agent to resolve the clinical outcomes observed in severe SARS-CoV-2 patients. PEGylated nanoparticle albumin-bound (PNAB) was used to promote prolonged bioactivity of steroidal ginsenoside saponins, PNAB-Rg6 and PNAB-Rgx365. Our data indicate that the application of PNAB-steroidal ginsenoside can effectively reduce histone H4 and NETosis-related factors in the plasma, and alleviate SREBP2-mediated systemic inflammation in the PBMCs of SARS-CoV-2 ICU patients. The engineered blood vessel model confirmed that these drugs are effective in suppressing blood clot formation and vascular inflammation. Moreover, the animal model experiment showed that these drugs are effective in promoting the survival rate by alleviating tissue damage and cytokine storm. Altogether, our findings suggest that these PNAB-steroidal ginsenoside drugs have potential applications in the treatment of symptoms associated with severe SARS-CoV-2 patients, such as coagulation and cytokine storm. English ELSEVIER SCI LTD EXTRACELLULAR HISTONES NANO-THERANOSTICS COVID-19 SEPSIS DEATH SHOCK TRAP PEGylated nanoparticle albumin-bound steroidal ginsenoside derivatives ameliorate SARS-CoV-2-mediated hyper-inflammatory responses Article 10.1016/j.biomaterials.2021.120827 1 BIOMATERIALS, v.273 BIOMATERIALS 273 scie scopus 000649670300002 2-s2.0-85105586919 Engineering, Biomedical Materials Science, Biomaterials Engineering Materials Science Article EXTRACELLULAR HISTONES NANO-THERANOSTICS COVID-19 SEPSIS DEATH SHOCK TRAP Albumin Ginsenoside COVID-19 NETosis Cytokine storm