Kwon, Ohman Jung, Kwang Bo Lee, Kyeong-Ryoon Son, Ye Seul Lee, Hana Kim, Jong-Jin Kim, Kwangho Lee, Seop Song, Yoo-Kyung Jung, Jaeeun Park, Kunhyang Kim, Dae-Soo Son, Myung Jin Lee, Mi-Ok Han, Tae-Su Cho, Hyun-Soo Oh, Soo Jin Chung, Haeun Kim, Sang-Heon Chung, Kyung-Sook Kim, Janghwan Jung, Cho-Rok Son, Mi-Young 2024-01-19T14:33:02Z 2024-01-19T14:33:02Z 2021-09-04 2021-06 2375-2548 https://pubs.kist.re.kr/handle/201004/116951 Advanced technologies are required for generating human intestinal epithelial cells (hIECs) harboring cellular diversity and functionalities to predict oral drug absorption in humans and study normal intestinal epithelial physiology. We developed a reproducible two-step protocol to induce human pluripotent stem cells to differentiate into highly expandable hIEC progenitors and a functional hIEC monolayer exhibiting intestinal molecular features, cell type diversity, and high activities of intestinal transporters and metabolic enzymes such as cytochrome P450 3A4 (CYP3A4). Functional hIECs are more suitable for predicting compounds metabolized by CYP3A4 and absorbed in the intestine than Caco-2 cells. This system is a step toward the transition from three-dimensional (3D) intestinal organoids to 2D hIEC monolayers without compromising cellular diversity and function. A physiologically relevant hIEC model offers a novel platform for creating patient-specific assays and support translational applications, thereby bridging the gap between 3D and 2D culture models of the intestine. English AMER ASSOC ADVANCEMENT SCIENCE PLURIPOTENT STEM-CELLS NOTCH WNT METABOLISM DIFFERENTIATION HOMEOSTASIS PROLIFERATION INHIBITION GENERATION SIGNALS The development of a functional human small intestinal epithelium model for drug absorption Article 10.1126/sciadv.abh1586 1 SCIENCE ADVANCES, v.7, no.23 SCIENCE ADVANCES 7 23 scie scopus 000658757100038 2-s2.0-85107300032 Multidisciplinary Sciences Science & Technology - Other Topics Article PLURIPOTENT STEM-CELLS NOTCH WNT METABOLISM DIFFERENTIATION HOMEOSTASIS PROLIFERATION INHIBITION GENERATION SIGNALS