Lee, Dong geun Jung, W.H. Lee, S. Yu, Eui Sang Lee, Taik jin KIM, JAE HUN Hyun, Seok Song LEE, KWAN HYI Lee, Seok Han, S.-K. Choi, M.C. Ahn, D.J. Ryu, Yong Sang Kim, Chulki 2024-01-19T14:33:07Z 2024-01-19T14:33:07Z 2021-09-02 2021-06 2041-1723 https://pubs.kist.re.kr/handle/201004/116956 Despite technological advances in biomolecule detections, evaluation of molecular interactions via potentiometric devices under ion-enriched solutions has remained a long-standing problem. To avoid severe performance degradation of bioelectronics by ionic screening effects, we cover probe surfaces of field effect transistors with a single film of the supported lipid bilayer, and realize respectable potentiometric signals from receptor？ligand bindings irrespective of ionic strength of bulky solutions by placing an ion-free water layer underneath the supported lipid bilayer. High-energy X-ray reflectometry together with the circuit analysis and molecular dynamics simulation discovered biochemical findings that effective electrical signals dominantly originated from the sub-nanoscale conformational change of lipids in the course of receptor？ligand bindings. Beyond thorough analysis on the underlying mechanism at the molecular level, the proposed supported lipid bilayer-field effect transistor platform ensures the world-record level of sensitivity in molecular detection with excellent reproducibility regardless of molecular charges and environmental ionic conditions. ？ 2021, The Author(s). English Nature Publishing Group Ionic contrast across a lipid membrane for Debye length extension: towards an ultimate bioelectronic transducer Article 10.1038/s41467-021-24122-8 1 Nature Communications, v.12, no.1 Nature Communications 12 1 Y scie scopus 000665038900009 2-s2.0-85108080625 Multidisciplinary Sciences Science & Technology - Other Topics Article ELECTRICAL DETECTION MOLECULAR-DYNAMICS DNA HYBRIDIZATION FIELD SENSORS AVIDIN TRANSISTORS DISEASE BINDING bioelectronic transducer lipid membrane Debye length molecular detection