Lee, Seul Gi Kim, Jin Soo Kim, Ha-Jeong Schlaepfer, David D. Kim, In-San Nam, Ju-Ock 2024-01-19T14:34:14Z 2024-01-19T14:34:14Z 2022-01-10 2021-05 2045-2322 https://pubs.kist.re.kr/handle/201004/117024 Several studies have suggested that extracellular matrix (ECM) remodeling and the microenvironment are tightly associated with adipogenesis and adipose angiogenesis. In the present study, we demonstrated that transforming growth factor-beta induced (TGFBI) suppresses angiogenesis stimulated by adipocyte-conditioned medium (Ad-CM), both in vitro and in vivo. TGFBI knockout (KO) mice exhibited increased numbers of blood vessels in adipose tissue, and blood vessels from these mice showed enhanced infiltration into Matrigel containing Ad-CM. The treatment of Ad-CM-stimulated SVEC-10 endothelial cells with TGFBI protein reduced migration and tube-forming activity. TGFBI protein suppressed the activation of the Src and extracellular signaling-related kinase signaling pathways of these SVEC-10 endothelial cells. Our findings indicated that TGFBI inhibited adipose angiogenesis by suppressing the activation of Src and ERK signaling pathways, possibly because of the stimulation of the angiogenic activity of endothelial cells. English Nature Publishing Group Endothelial angiogenic activity and adipose angiogenesis is controlled by extracellular matrix protein TGFBI Article 10.1038/s41598-021-88959-1 1 Scientific Reports, v.11, no.1 Scientific Reports 11 1 Y scie scopus 000656986200001 2-s2.0-85105459099 Multidisciplinary Sciences Science & Technology - Other Topics Article TISSUE ANGIOGENESIS REGIONAL DIFFERENCES GENE-EXPRESSION CELL-ADHESION OBESITY GROWTH BETA-IG-H3 FIBROSIS MICE