Kim, Do-Geun Kim, Hyeon-Joong Choi, Sun-Hye Nam, Sung Min Kim, Hyoung-Chun Rhim, Hyewhon Cho, Ik-Hyun Rhee, Man Hee Nah, Seung-Yeol 2024-01-19T14:34:32Z 2024-01-19T14:34:32Z 2021-09-05 2021-05 1226-8453 https://pubs.kist.re.kr/handle/201004/117044 Background: Gintonin is an exogenous ginseng-derived G-protein-coupled lysophosphatidic acid (LPA) receptor ligand. LPA induces in vitro morphological changes and migration through neuronal LPA1 receptor. Recently, we reported that systemic administration of gintonin increases blood-brain barrier (BBB) permeability via the paracellular pathway and its binding to brain neurons. However, little is known about the influences of gintonin on in vivo neuron morphology and migration in the brain. Materials and methods: We examined the effects of gintonin on in vitro migration and morphology using primary hippocampal neural precursor cells (hNPC) and in vivo effects of gintonin on adult brain neurons using real time microscopic analysis and immunohistochemical analysis to observe the morphological and locational changes induced by gintonin treatment. Results: We found that treating hNPCs with gintonin induced morphological changes with a cell rounding following cell aggregation and return to individual neurons with time relapses. However, the in vitro effects of gintonin on hNPCs were blocked by the LPA1/3 receptor antagonist, Ki16425, and Rho kinase inhibitor, Y27632. We also examined the in vivo effects of gintonin on the morphological changes and migration of neurons in adult mouse brains using anti-NeuN and -neurofilament H antibodies. We found that acute intravenous administration of gintonin induced morphological and migrational changes in brain neurons. Gintonin induced some migrations of neurons with shortened neurofilament H in the cortex. The in vivo effects of gintonin were also blocked by Ki16425. Conclusion: The present report raises the possibility that gintonin could enter the brain and exert its influences on the migration and morphology of adult mouse brain neurons and possibly explains the therapeutic effects of neurological diseases behind the gintonin administration. (C) 2020 The Korean Society of Ginseng. Publishing services by Elsevier B.V. English 고려인삼학회 Gintonin influences the morphology and motility of adult brain neurons via LPA receptors Article 10.1016/j.jgr.2020.06.003 1 Journal of Ginseng Research, v.45, no.3, pp.401 - 407 Journal of Ginseng Research 45 3 401 407 Y scie scopus kci ART002724203 000652190700005 2-s2.0-85090487616 Plant Sciences Chemistry, Medicinal Integrative & Complementary Medicine Plant Sciences Pharmacology & Pharmacy Integrative & Complementary Medicine Article LYSOPHOSPHATIDIC ACID RECEPTOR MICE LIGAND Gintonin Morphology and migration LPA receptors Adult brain neuron