Hong, Subeen Park, Yehyon Kim, Yu Mi Lee, Ji Eun Kim, Hyeon Ji Park, Kyo Hoon 2024-01-19T15:01:11Z 2024-01-19T15:01:11Z 2021-09-02 2021-05 1046-7408 https://pubs.kist.re.kr/handle/201004/117111 Problem We aimed to identify amniotic fluid (AF) proteins associated with the subsequent rupture of membranes (ROM) occurring in the absence of active labor in women with threatened preterm labor (PTL) using an antibody microarray. Method of the study This retrospective cohort study included 183 singleton pregnant women with PTL (24-33 weeks) who underwent amniocentesis. A nested case-control study was conducted using AF samples from 20 women with subsequent ROM within 7 days of sampling (case subjects) and 20 gestational age-matched women with term delivery (TD) without ROM (control subjects), via protein-antibody microarray analysis. Seven candidate proteins of interest were validated via ELISA in the total cohort. Results Seventeen proteins displayed significant intergroup differences. ELISA validation confirmed that the levels of EN-RAGE, Fas, IL-8, IP-10, MMP-8, and MMP-9 were significantly higher, whereas IGFBP-3 levels were significantly lower in the AF of women with subsequent ROM within 7 days of sampling than in that of women with TD without ROM. Moreover, the time interval from sampling to membrane rupture was significantly correlated with the expression levels of AF proteins, except for IL-10. Conclusion Using an antibody microarray, we identified various inflammatory, angiogenic, matrix-degrading, and apoptosis-related proteins in the AF that were associated with subsequent ROM occurring in the absence of active labor in women with threatened PTL. These findings provide novel insights into the molecular mechanisms underlying membrane rupture without active labor in threatened PTL. English WILEY FACTOR-BINDING PROTEIN-3 PREMATURE RUPTURE MATRIX METALLOPROTEINASES CYTOKINE LEVELS GROWTH PLACENTA RISK EPIDEMIOLOGY INFECTION APOPTOSIS Antibody microarray analysis of amniotic fluid proteins associated with subsequent ruptured membranes in women with threatened preterm labor Article 10.1111/aji.13371 1 AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY, v.85, no.5 AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY 85 5 scie scopus 000591858500001 2-s2.0-85096710315 Immunology Reproductive Biology Immunology Reproductive Biology Article FACTOR-BINDING PROTEIN-3 PREMATURE RUPTURE MATRIX METALLOPROTEINASES CYTOKINE LEVELS GROWTH PLACENTA RISK EPIDEMIOLOGY INFECTION APOPTOSIS amniotic fluid antibody microarray preterm labor proteins ruptured membranes