Park, Sang Eun Yeon, Gyu-Bum Goo, Hui-Gwan Seo, Dong Sik Dayem, Ahmed A. Lee, Kyung Eun Park, Hyun-Mee Cho, Ssang-Goo Kim, Dae-Sung 2024-01-19T15:01:12Z 2024-01-19T15:01:12Z 2021-09-02 2021-05 0021-9541 https://pubs.kist.re.kr/handle/201004/117112 Polyvinylidene fluoride (PVDF) is biocompatible, easy to fabricate, and has piezoelectric properties; it has been used for many biomedical applications including stem cell engineering. However, long-term cultivation of human embryonic stem cells (hESCs) and their differentiation toward cardiac lineages on PVDF have not been investigated. Herein, PVDF nanoscaled membrane scaffolds were fabricated by electrospinning; a vitronectin-derived peptide-mussel adhesive protein fusion (VNm) was immobilized on the scaffolds. hESCs cultured on the VNm-coated PVDF scaffold (VNm-PVDF scaffold) were stably expanded for more than 10 passages while maintaining the expression of pluripotency markers and genomic integrity. Under cardiac differentiation conditions, hESCs on the VNm-PVDF scaffold generated more spontaneously beating colonies and showed the upregulation of cardiac-related genes, compared with those cultured on Matrigel and VNm alone. Thus, VNm-PVDF scaffolds may be suitable for the long-term culture of hESCs and their differentiation into cardiac cells, thus expanding their application in regenerative medicine. English John Wiley & Sons Inc. Maintenance and differentiation of human ES cells on polyvinylidene fluoride scaffolds immobilized with a vitronectin-derived peptide Article 10.1002/jcp.30095 1 Journal of Cellular Physiology, v.236, no.5, pp.3510 - 3520 Journal of Cellular Physiology 236 5 3510 3520 N scie scopus 000576153200001 2-s2.0-85092200171 Cell Biology Physiology Cell Biology Physiology Article ADHESION PVDF BIOMATERIAL NANOFIBERS BEHAVIOR cardiac differentiation human embryonic stem cells long-term culture polyvinylidene fluoride scaffolds