Suhito, Intan Rosalina Han, Yoojoong Ryu, Yong-Sang Son, Hyungbin Kim, Tae-Hyung 2024-01-19T15:01:25Z 2024-01-19T15:01:25Z 2022-01-10 2021-04-15 0956-5663 https://pubs.kist.re.kr/handle/201004/117124 Stem cell-based therapies have recently emerged to treat various incurable diseases and disorders. Types of stem cell-derived cells and their functions should be intensively analyzed before therapy. However, current pretreatment steps for biological analysis are mostly destructive. Here, we report a novel optical method that enables ultra-fast and label-free characterization of cells, eliminating invasive, destructive steps. The technique, referred to as "autofluorescence-Raman mapping integration (ARMI)" analysis uses cell autofluorescence (AF) to reveal cellular morphology and cytosolic microstructures, while Raman mapping allows site-specific intensive analysis of target molecules, which enables ultra-fast identification of cell types. We used human mesenchymal stem cells (MSCs) as a model and induced adipogenesis. Lipid droplets in cells appeared as "blanks" in three-dimensional AF images and site-specific Raman mapping guided by AF identified the structure and components of the CH2 stretch. Adipogenesis could be rapidly and precisely analyzed, not only for the same batch but also for different batches. Therefore, the developed tool is highly useful for the accurate screening of stem cell differentiation and implementation in biomedical and clinical applications. English ELSEVIER ADVANCED TECHNOLOGY Autofluorescence-Raman Mapping Integration analysis for ultra-fast label-free monitoring of adipogenic differentiation of stem cells Article 10.1016/j.bios.2021.113018 1 BIOSENSORS & BIOELECTRONICS, v.178 BIOSENSORS & BIOELECTRONICS 178 scie scopus 000621208000004 2-s2.0-85099941250 Biophysics Biotechnology & Applied Microbiology Chemistry, Analytical Electrochemistry Nanoscience & Nanotechnology Biophysics Biotechnology & Applied Microbiology Chemistry Electrochemistry Science & Technology - Other Topics Article PLURIPOTENCY SPECTROSCOPY METABOLISM STRATEGIES DENSITY Autofluorescence microscopy Raman spectroscopy Stem cells Differentiation variability Label-free method