So, In-Seop Kang, Jin Hee Hong, Jung Wan Sung, Shijin Hasan, Al Faruque Sa, Keum Hee Han, Seung Woo Kim, In San Kang, Young Mo 2024-01-19T15:02:28Z 2024-01-19T15:02:28Z 2022-01-10 2021-04 1360-8185 https://pubs.kist.re.kr/handle/201004/117175 Apoptosis plays an essential role in the pathophysiologic processes of rheumatoid arthritis. A molecular probe that allows spatiotemporal observation of apoptosis in vitro, in vivo, and ex vivo concomitantly would be useful to monitoring or predicting pathophysiologic stages. In this study we investigated whether cyclic apoptosis-targeting peptide-1 ((C)ApoPep-1) can be used as an apoptosis imaging probe in inflammatory arthritis. We tested the utility of (C)ApoPep-1 for detecting apoptotic immune cells in vitro and ex vivo using flow cytometry and immunofluorescence. The feasibility of visualizing and quantifying apoptosis using this probe was evaluated in a murine collagen-induced arthritis (CIA) model, especially after treatment. (C)ApoPep-1 peptide may successfully replace Annexin V for in vitro and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay for ex vivo in the measurement of apoptotic cells, thus function as a sensitive probe enough to be used clinically. In vivo imaging in CIA mice revealed that (C)ApoPep-1 had 42.9 times higher fluorescence intensity than Annexin V for apoptosis quantification. Furthermore, it may be used as an imaging probe for early detection of apoptotic response in situ after treatment. The (C)ApoPep-1 signal was mostly co-localized with the TUNEL signal (69.6% of TUNEL+ cells) in defined cell populations in joint tissues of CIA mice. These results demonstrate that (C)ApoPep-1 is sufficiently sensitive to be used as an apoptosis imaging probe for multipurpose applications which could detect the same target across in vitro, in vivo, to ex vivo in inflammatory arthritis. English SPRINGER A novel apoptosis probe, cyclic ApoPep-1, for in vivo imaging with multimodal applications in chronic inflammatory arthritis Article 10.1007/s10495-021-01659-z 1 APOPTOSIS, v.26, no.3-4, pp.209 - 218 APOPTOSIS 26 3-4 209 218 scie scopus 000624434400001 2-s2.0-85101872497 Biochemistry & Molecular Biology Cell Biology Biochemistry & Molecular Biology Cell Biology Article Cyclic ApoPep-1 Probe Apoptosis imaging Rheumatoid arthritis Multipurpose applications