Kim, Seongchan Park, Sungwook Cho, Young Soo Kim, Younghoon Tae, Jong Hyun Il No, Tae Shim, Ji Sung Jeong, Youngdo Kang, Seok Ho Lee, Kwan Hyi 2024-01-19T15:03:52Z 2024-01-19T15:03:52Z 2021-09-05 2021-03-26 2379-3694 https://pubs.kist.re.kr/handle/201004/117246 Urinary miRNAs are biomarkers that demonstrate considerable promise for the noninvasive diagnosis and prognosis of diseases. However, because of background noise resulting from complex physiological features of urine, instability of miRNAs, and their low concentration, accurate monitoring of miRNAs in urine is challenging. To address these limitations, we developed a urine-based disposable and switchable electrical sensor that enables reliable and direct identification of miRNAs in patient urine. The proposed sensing platform combining disposable sensor chips composed of a reduced graphene oxide nanosheet and peptide nucleic acid facilitates the label-free detection of urinary miRNAs with high specificity and sensitivity. Using real-time detection of miRNAs in patient urine without pretreatment or signal amplification, this sensor allows rapid, direct detection of target miRNAs in a broad dynamic range with a detection limit down to 10 fM in human urine specimens within 20 min and enables simultaneous quantification of multiple miRNAs. As confirmed using a blind comparison with the results of pathological examination of patients with prostate cancer, the sensor offers the potential to improve the accuracy of early diagnosis before a biopsy is taken. This study holds the usefulness of the practical sensor for the clinical diagnosis of urological diseases. English AMER CHEMICAL SOC Electrical Cartridge Sensor Enables Reliable and Direct Identification of MicroRNAs in Urine of Patients Article 10.1021/acssensors.0c01870 1 ACS Sensors, v.6, no.3, pp.833 - 841 ACS Sensors 6 3 833 841 scie scopus 000635484500027 2-s2.0-85097801694 Chemistry, Multidisciplinary Chemistry, Analytical Nanoscience & Nanotechnology Chemistry Science & Technology - Other Topics Article FIELD-EFFECT-TRANSISTOR PROSTATE-CANCER GRAPHENE-OXIDE ONE-STEP NANOPARTICLES BIOMARKERS ANTISENSE SYSTEM MIRNA GENE clinical diagnosis prostate cancer urinary miRNA detection disposable sensor chips graphene-based electrical sensor label-free detection patient urine